Evaluation of Formalin Fixation for Tissue Biopsies Using Shear Wave Laser Speckle Imaging System.
Evaluation of Formalin Fixation for Tissue Biopsies Using Shear Wave Laser Speckle Imaging System.
复制标题
使用剪切波激光散斑成像系统评估福尔马林固定组织活检的效果。
DOI：
10.1109/jtehm.2019.2909914
复制
发表时间：
2019
影响因子：
3.4
通讯作者：
Seibel,EricJ
中科院分区：
文献类型：
--
作者：
Lim,SanielD;Huang,Qixuan;Seibel,EricJ
化学固定是组织病理学准备组织的多步骤过程中最慢且通常最不受控制的步骤。为了减少从进行芯针活检到做出诊断的时间，提出了一种新的方法，该方法光学监测常见的福尔马林固定过程。提出了一种低成本、高灵敏度的激光散斑成像技术，用于测量浸没在毫米流体通道中的厚度为0.5mm的生物样品中的剪切波速度。利用明胶体模、鸡胸肉以及固定后的人体肝脏和结肠组织，测量了压电致动产生的剪切波速度，该速度是组织力学性能的指标。明胶体模和鸡胸肉中剪切波速度的固定水平分别增加了约271.0%和130.8%，然后以10.91 m/s和7.88 m/s的速度达到平台。在这些小样本中，平台水平和时间随测量位置而变化，并且在明胶和鸡胸之间变化。这种基于光学的方法证明了微调预分析变量的可行性，例如固定时间，用于快速准确的组织病理学评估;在大多数病理学实验室进行的组织制备方案期间提供质量度量;并引入了可以设计为未来一次性设备的毫米流体室，该设备可以自动进行活检处理和成像。
Chemical fixation is the slowest and often the most uncontrolled step in the multi-step process of preparing tissue for histopathology. In order to reduce the time from taking a core needle biopsy to making a diagnosis, a new approach is proposed that optically monitors the common formalin fixation process. A low-cost and highly-sensitive laser speckle imaging technique is developed to measure shear wave velocity in a biospecimen as small as 0.5 mm in thickness submerged in millifluidic channels. Shear wave velocity, which is the indicator of tissue mechanical property and induced by piezoelectric-actuation, was monitored using gelatin phantom and chicken breast during fixation, as well as post-fixed liver and colon tissues from human. Fixation levels in terms of shear wave velocity increased by approximately 271.0% and 130.8% in gelatin phantom and chicken breast, respectively, before reaching the plateaus at 10.91 m/s and 7.88 m/s. Within these small specimens, the plateaus levels and times varied with location of measurement, and between gelatin and chicken breast. This optical-based approach demonstrates the feasibility of fine-tuning preanalytical variables, such as fixation time, for a rapid and accurate histopathological evaluation; provides a quality metric during the tissue preparation protocol performed in most pathology labs; and introduces the millifluidic chamber that can be engineered to be a future disposable device that automates biopsy processing and imaging.