Photodynamic Therapy Using Indocyanine Green Loaded on Super Carbonate Apatite as Minimally Invasive Cancer Treatment
Photodynamic Therapy Using Indocyanine Green Loaded on Super Carbonate Apatite as Minimally Invasive Cancer Treatment
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DOI：
10.1158/1535-7163.mct-17-0788
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发表时间：
2018-04
影响因子：
5.7
通讯作者：
Koki Tamai;T. Mizushima;Xin Wu;Akira Inoue;Minori Ota;Yuhki Yokoyama;N. Miyoshi;N. Haraguchi;H. Takahashi;J. Nishimura;T. Hata;C. Matsuda;Y. Doki;M. Mori;Hirofumi Yamamoto
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作者：
Koki Tamai;T. Mizushima;Xin Wu;Akira Inoue;Minori Ota;Yuhki Yokoyama;N. Miyoshi;N. Haraguchi;H. Takahashi;J. Nishimura;T. Hata;C. Matsuda;Y. Doki;M. Mori;Hirofumi Yamamoto
在老龄化社会中，微创治疗变得越来越重要。本研究的目的是探讨负载ICG的超碳酸盐磷灰石（sCA）纳米粒子作为一种新的光动力学治疗（PDT）的癌症的可能性。使用结肠癌细胞，在ICG和sCA-ICG处理之间检查ICG摄取和抗肿瘤作用。活性氧（ROS）的产生和温度上升也进行了评估，以探讨潜在的机制。原子力显微镜显示，sCA-ICG的大小范围为10至20 nm。在含有0.5%白蛋白的水溶液中，sCA-ICG和ICG中激光照射后的温度升高分别为27.1°C和23.1°C（对照DW：5.7°C）。sCA-ICG+照射组细胞ROS生成量明显高于ICG+照射组（P < 0.01）。在体外和体内，与ICG相比，sCA-ICG中肿瘤细胞对ICG的摄取显著增加。在两种治疗中，肿瘤、肝脏和肾脏中ICG的荧光信号在24小时内消失。最后，用sCA-ICG处理的HT 29肿瘤随后照射显示出显著的肿瘤生长抑制（P < 0.01），而在注射ICG后照射肿瘤不抑制肿瘤生长。这项研究表明，sCA是一个有用的工具ICG为基础的PDT。ICG从正常器官中的快速撤回是sCA-ICG所独有的，并且与长时间保留在正常器官中的其他纳米颗粒形成对比。Mol Cancer Ther; 17（7）; 1613-22.©2018 AACR.
Minimally invasive treatment is getting more and more important in an aging society. The purpose of this study was to explore the possibility of ICG loaded on super carbonate apatite (sCA) nanoparticles as a novel photodynamic therapy (PDT) against cancers. Using colon cancer cells, ICG uptake and anti-tumor effects were examined between the treatments of ICG and sCA-ICG. Reactive oxygen species (ROS) production and temperature rise were also evaluated to explore the underlying mechanism. Atomic force microscopy revealed that the size of sCA-ICG ranged from 10 to 20 nm. In aqueous solution with 0.5% albumin, the temperature increase after laser irradiation was 27.1°C and 23.1°C in sCA-ICG and ICG, respectively (control DW: 5.7°C). A significant increase in ROS generation was noted in cell cultures treated with sCA-ICG plus irradiation compared with those treated with ICG plus irradiation (P < 0.01). Uptake of ICG in the tumor cells significantly increased in sCA-ICG compared with ICG in vitro and in vivo. The fluorescence signals of ICG in the tumor, liver, and kidney faded away in both treatments by 24 hours. Finally, the HT29 tumors treated with sCA-ICG followed by irradiation exhibited drastic tumor growth retardation (P < 0.01), whereas irradiation of tumors after injection of ICG did not inhibit tumor growth. This study shows that sCA is a useful vehicle for ICG-based PDT. Quick withdrawal of ICG from normal organs is unique to sCA-ICG and contrasts with the other nanoparticles remaining in normal organs for a long time. Mol Cancer Ther; 17(7); 1613–22. ©2018 AACR.