Birth weight, umbilical and placental traits in relation to neonatal loss in cloned pigs
Birth weight, umbilical and placental traits in relation to neonatal loss in cloned pigs
复制标题
DOI：
10.1016/j.placenta.2017.06.010
复制
发表时间：
2017-09-01
期刊：
影响因子：
3.8
通讯作者：
Wu, Zhenfang
中科院分区：
文献类型：
--
作者：
Ao, Zheng;Liu, Dewu;Wu, Zhenfang
体细胞核移植(SCNT)产生的克隆猪具有较高的新生儿死亡率。在受精衍生的小猪中，出生后损失与低出生体重、脐带状况和胎盘参数有关。为了研究克隆仔猪是否也存在这种关系，比较了四组仔猪的出生体重、脐带状况、胎盘参数、胎盘形态和基因表达谱，即SCNT-DW4组(SCNT-DW4)、SCNT-DW4组(SCNT-SO4)、人工授精(AI-DW4)组(AI-DW4)和铝系仔猪组(AI-SO4)。结果表明，SCNT-DW4仔猪脐带异常发生率显著高于AI-SO4仔猪，但与SCNT-SO4和AI-DW4仔猪相似。AI-SO4组、AI-DW4组和SCNT-SO4组的出生体重、胎盘表面积和胎盘重量相似，但显著高于SCNT-DW4组。与Al-SO4相比，SCNT-SO4胎盘表现出轻微的形态异常，而SCNT-DW4胎盘表现出严重的形态异常。与SCNT-SO4和Al-SO4胎盘相比，SCNT-DW4胎盘中印迹、血管生成、营养物质转运、细胞凋亡和氧化应激相关基因的表达谱存在错误，两者具有相似的基因表达谱。这些结果表明，出生体重、脐带状况、胎盘参数、胎盘形态和基因表达与克隆仔猪的新生儿死亡有关。克隆仔猪在新生期的高失败率可能是由于产前胚胎外发育严重不足所致。(C)2017爱思唯尔有限公司。保留所有权利。
Cloned piglets generated through somatic cell nuclear transfer (SCNT) have a high rate of neonatal death. Postnatal loss is associated with low birth weight, umbilical status and placental parameters in fertilisation-derived piglets. To investigate whether or not this relationship also exists in cloned piglets, birth weight, umbilical status, placental parameters, placental morphology and gene expression pattern were compared among four piglet groups, namely, SCNT-derived male piglets that died within 4 days (SCNT-DW4), SCNT-derived male piglets that survived over 4 days (SCNT-SO4), artificial insemination (AI)-generated male piglets that died within 4 days (AI-DW4) and Al-generated male piglets that survived over 4 days (AI-SO4). Results showed that the occurring frequency of abnormal umbilical cord in SCNT-DW4 piglets was significantly higher than that in AI-SO4 piglets but was similar to that in SCNT-SO4 and AI-DW4 piglets. The birth weight, placental surface area and placental weight of AI-SO4, AI-DW4 and SCNT-SO4 groups were similar but were significantly higher than those in SCNT-DW4 group. SCNT-SO4 placentas exhibited mild but SCNT-DW4 placentas showed severe morphological abnormalities compared with Al-SO4 placentas. The expression profiles of imprinting, angiopoiesis, nutrient transport, apoptosis and oxidative stress-related genes in SCNT-DW4 placentas were erroneous compared with those in SCNT-SO4 and Al-SO4 placentas, which both had similar gene expression patterns. These results indicate that birth weight, umbilical status, placental parameters, placental morphology and gene expression were associated with neonatal death of cloned piglets. The high loss of cloned piglets during neonatal age may be caused by severe deficiency of extra-embryonic development during prenatal stage. (C) 2017 Elsevier Ltd. All rights reserved.