Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells.
Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells.
复制标题
在胎儿和肿瘤细胞中表达增强的人类核糖体蛋白基因的克隆和表征。
DOI：
10.1093/nar/15.21.8919
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发表时间：
1987
影响因子：
14.9
通讯作者：
Rutter,WJ
中科院分区：
文献类型：
--
作者：
Ou,JH;Yen,TS;Wang,YF;Kam,WK;Rutter,WJ
肝细胞癌与B型肝炎病毒携带者密切相关，这些患者通常具有整合在肝细胞染色体DNA中的HBV序列。为了评估HBV调控序列（例如，增强子）对邻近宿主基因表达的影响我们已经筛选了在肝细胞癌细胞中过表达和邻近整合的HBV序列的细胞基因。其中一个基因的克隆cDNA编码一种类似于E. coli L-3核糖体蛋白，其被认为在mRNA与核糖体的结合中起作用。由cDNA编码的蛋白定位于核仁，也存在于核糖体中;可能是L-3（MRL 3）的哺乳动物同源物。MRL 3在结肠癌和淋巴瘤细胞系中的表达高于正常肝脏、胎盘和二倍体成纤维细胞，在胎儿肝脏中的表达也高于成人肝脏。因此，MRL 3过表达似乎是快速分裂细胞的特性，与肿瘤发生没有直接联系。
Hepatocellular carcinoma is strongly associated with hepatitis B virus carrier patients who usually have HBV sequences integrated in the chromosomal DNA of liver cells. To assess the possible effects of HBV regulatory sequences (e.g., the enhancer) on expression of neighboring host genes we have screened for cellular genes that are both overexpressed and adjacent to integrated HBV sequences in hepatocellular carcinoma cells . The cloned cDNA for one such gene encodes a protein similar to the E. coli L-3 ribosomal protein which is thought to play a role in mRNA binding to the ribosome. The protein encoded by the cDNA localizes to the nucleolus and is also found in ribosomes; possibly it is the mammalian homologue of L-3 (MRL3). The expression of MRL3 is higher in colon carcinoma and lymphoma cell lines than in normal liver , placenta and diploid fibroblasts, and is also higher in fetal than in adult liver. Therefore, MRL3 overexpression seems to be a property of rapidly dividing cells and is not directly linked to oncogenesis.