FAR-RED ELONGATED HYPOCOTYLS3 negatively regulates shade avoidance responses in Arabidopsis
FAR-RED ELONGATED HYPOCOTYLS3 negatively regulates shade avoidance responses in Arabidopsis
复制标题
远红细长下胚轴3负向调节拟南芥的避荫反应
DOI：
10.1111/pce.13630
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Qin Xiaochun
中科院分区：
文献类型：
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作者：
Ma Lin;Li Yang;Li Xiuxiu;Xu Di;Lin Xueqiao;Liu Mingmei;Li Gang;Qin Xiaochun
Light is a key limiting factor of plant growth and development under the canopy. Specific light signals, such as a low ratio of red : far‐red (R:FR) light, trigger the shade avoidance response, which affects hypocotyl, stem, and leaf growth. Although multiple components mediating shade avoidance responses have been identified in the past few decades, the underlying regulatory mechanism remains unclear. In this study, we found that thefar‐red elongated hypocotyls 3(fhy3) mutant exhibited longer hypocotyls and increased expression levels of core shade avoidance response genes under low R:FR shade conditions compared with the wild type No‐0, suggesting that FHY3 negatively regulates shade avoidance responses. Yeast one‐hybrid, chromatin immunoprecipitation, and RT‐qPCR assays revealed that FHY3 directly binds to the promoters and gene body ofPHYTOCHROME RAPIDLY REGULATED1(PAR1) andPAR2and activates their expression to inhibit shade responses. Furthermore, the overexpression ofPAR1orPAR2rescued the enhanced shade avoidance responses offhy3, indicating that both genes are direct downstream targets of FHY3 that mediate shade avoidance responses. Our findings demonstrate that the light‐signalling protein FHY3 positively regulates the transcription ofPAR1andPAR2, which encode two key negative regulators of shade avoidance responses, thus repressing plant responses to shade signals.
