Glutamate receptor subunits expression in memory-associated brain structures: Regional variations and effects of aging
Glutamate receptor subunits expression in memory-associated brain structures: Regional variations and effects of aging
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DOI：
10.1002/syn.20563
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发表时间：
2008-11-01
期刊：
影响因子：
2.3
通讯作者：
Darlington, Cynthia L.
中科院分区：
文献类型：
--
作者：
Liu, Ping;Smith, Paul F.;Darlington, Cynthia L.
我们用Western blotting研究了N-甲基-D-天冬氨酸(NMDA)和α-氨基-3-羟基-5-甲基-4-异恶唑(AMPA)受体亚基在几个记忆相关结构中的区域差异和衰老对其表达的影响。在年轻的成年大鼠中，NR1、NR2A和GluR2的水平在海马区和海马区之间以及海马亚区之间变化。在青年(4月龄)和老年(24月龄)大鼠之间进行比较时，发现NR1在老年大鼠的腹侧海马区以及嗅内和嗅后皮质中的表达显著降低。NR2A在老年大鼠海马区的表达明显降低，但在海马区的表达无明显变化。G1uR2亚单位在腹侧海马区和纹状体后皮质的表达显著降低。老年CA2/3组和CA1组NR1和G1uR2的表达显著降低，而NR2a的表达无明显增龄变化。在两个年龄组的前额叶皮质中，这三个亚单位的表达水平相似。这些结果提示记忆相关脑结构中NMDA和AMPA受体亚单位的差异表达和衰老的影响。
We investigated regional variations and the effects of aging on the expression of the N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) receptor subunits in several memory-associated structures using Western blotting. In young adult rats, NR1, NR2A, and GluR2 levels varied between the hippocampus and parahippocampal region and between the subregions of the hippocampus. When a comparison was made between young (4-month-old) and aged (24-month-old) rats, significant decreases in NR1 expression were found in the aged ventral hippocampus and the entorhinal and postrhinal cortices. There were significant decreases in NR2A expression in the aged parahippocampal region, but not in the hippocampus. The expression of the G1uR2 subunit was significantly reduced in the ventral hippocampus and the postrhinal cortex. A dramatic decrease in NR1 and G1uR2 expression was found in the aged CA2/3 and CA1, respectively, but there were no significant age-related changes in NR2A expression. All three subunits were expressed at a similar level in the two age groups in the prefrontal cortex. These results suggest differential expression and effects of aging on NMDA and AMPA receptor subunits in memory-associated brain structures.