A simple method for the determination of polycyclic aromatic hydrocarbons (pah) in edible oil employing solid phase extraction (SPE) cartridge purification
A simple method for the determination of polycyclic aromatic hydrocarbons (pah) in edible oil employing solid phase extraction (SPE) cartridge purification
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DOI：
10.1007/s10068-013-0073-1
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发表时间：
2013-02-01
影响因子：
2.9
通讯作者：
Chae, Young-Zoo
中科院分区：
文献类型：
--
作者：
Jung, So-Young;Park, Ju-Sung;Chae, Young-Zoo
本文报道了一种简单、快速且廉价的测定食用油中多环芳烃 (PAH) 的方法。将样品溶解在溶剂中，并将一部分溶液加载到固相萃取柱上。用异辛烷/环己烷混合物洗去柱中的油，并用己烷/二氯甲烷混合物洗脱吸收的PAH。采用气相色谱-串联质谱仪分析了芝麻油和紫苏油中的 15 种欧盟优先考虑的多环芳烃。分析限(LOD)为0.01-0.06μg/kg，定量限(LOQ)为0.03-0.17μg/kg，回收率范围为55.1-105.0%。该方法使用有证标准物质 (CRM) 进行验证，误差值范围为 2.06-4.30%。对二十一个商业样品进行了分析，以确定其多环芳烃污染水平。商业样品中15种PAHs的总浓度范围为未检出至9.627μg/kg。仅1个样品苯并(a)芘含量超标，超过2.0μg/kg。
This paper reports a simple, fast, and inexpensive method for the determination of polycyclic aromatic hydrocarbons (PAH) in edible oils. The sample was dissolved in solvents, and a portion of the solution was loaded onto a solid phase extraction cartridge. The oil from the cartridge was washed away by isooctane/cyclohexane mixture and the absorbed PAHs were eluted with hexane/ dichloromethane mixture. GC-tandem MS was used to analyze 15 PAHs of EU priority in sesame oil and perilla oil. Analytical limits of determination (LOD) were 0.01-0.06 mu g/kg, limits of quantitation (LOQ) were 0.03-0.17 mu g/kg, and the recovery values ranged 55.1-105.0%. This method was validated using the certified reference material (CRM) and the error values ranged 2.06-4.30%. Twentyone commercial samples were analyzed to determine their PAHs contamination levels. The total concentration of the 15 PAHs in commercial samples ranged from not detected to 9.627 mu g/kg. Only 1 sample had excessive benzo(a)pyrene content, which was more than 2.0 mu g/kg.