Constitutive Expression of Interleukin‐7 mRNA and Production of IL‐7 by a Cloned Murine Thymic Stromal Cell Line
Constitutive Expression of Interleukin‐7 mRNA and Production of IL‐7 by a Cloned Murine Thymic Stromal Cell Line
复制标题
克隆鼠胸腺基质细胞系白细胞介素 7 mRNA 的组成型表达和 IL-7 的产生
DOI：
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发表时间：
1990
影响因子：
5.5
通讯作者：
H. Fujiwara
中科院分区：
文献类型：
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作者：
T. Sakata;S. Iwagami;Y. Tsuruta;H. Teraoka;Y. Tatsumi;Yasumichi Kita;S. Nishikawa;Y. Takai;H. Fujiwara
利用聚合酶链式反应(PCR)技术从小鼠胸腺基质细胞克隆MRL104.8a中克隆了小鼠白细胞介素7(IL-7)基因，并在COS-7细胞中表达。得到的重组白介素7(rll-7)在没有IL-2和抗原的情况下支持小鼠抗原特异性辅助T细胞(Th)克隆9-16的增殖，并支持小鼠前B细胞系DW34的增殖。研究还发现，MRL104.8a细胞中存在高水平的IL-7mRNA的组成性表达，并在其培养上清液中产生大量的IL-7。这些结果为胸腺基质细胞表达IL-7mRNA和产生IL-7提供了证据，而胸腺基质细胞在胸腺内T细胞发育中起着关键作用。这些结果在IL-7和先前描述的由胸腺基质细胞产生的细胞因子之间的功能和分子关系的背景下进行了讨论。
Mouse interleukin‐7 (IL‐7) cDNA was cloned from mouse thymic stromal cell clone MRL104.8a using a polymerase chain reaction (PCR) technique and expressed in COS‐7 cells. The resulting recombinant interleukin‐7 (rlL‐7) supported the proliferation of mouse antigen‐specific helper T cell (Th) clone 9–16 in the absence of IL‐2 and antigen as well as mouse pre‐B cell line DW34. It was also found that high levels of the mRNA for IL‐7 were constitutively expressed in the MRL104.8a cells, and a potent amount of IL‐7 was produced in its culture supernatant. These results provide the evidence for constitutive expression of IL‐7 mRNA and for production of IL‐7 by thymic stromal cells that have a critical role in intrathymic T cell development. The results are discussed in the context of the functional and molecular relationship between IL‐7 and the previously described cytokines produced by thymic stromal cells.