Proteomic analysis of Magnolia sieboldii K. Koch seed germination
Proteomic analysis of Magnolia sieboldii K. Koch seed germination
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天女木兰种子萌发的蛋白质组学分析
DOI：
10.1016/j.jprot.2015.12.005
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发表时间：
2016-02-05
影响因子：
3.3
通讯作者：
Ma, Bei-bei
中科院分区：
文献类型：
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作者：
Lu, Xiu-jun;Zhang, Xiao-lin;Ma, Bei-bei
天女木兰是原产于中国的落叶乔木。本种具有深休眠特性。为了更好地了解种子萌发，我们使用蛋白质分析的种子蛋白质的变化在0，65，110和150天的层积。比较2DE分析M.结果表明，在0、65、110和150 d层积时，天女木兰种子蛋白质谱显示出80种不同丰度的蛋白质。比较分析表明，ADP-葡萄糖焦磷酸化酶小亚基在萌发过程中发生降解。特别是，它几乎完全降解，在110 d的发芽。层积65 d后，微观结构中的淀粉粒减少。淀粉颗粒为随后的萌发提供了足够量的底物和ATP。共鉴定出4种贮藏蛋白，均为向下积累蛋白。斑点44和46具有不同的分子量和pI值，斑点36和46具有几乎相同的分子量，但在2-DE凝胶中pI发生了变化，这表明它们可能是同一蛋白家族的不同异构体和翻译后修饰。我们的结果表明，淀粉颗粒和贮藏蛋白种类的降解为种子胚的生长做好准备，并调节种子萌发。目前的蛋白质组学分析提供了新的见解，动员的营养储备在萌发的M。生物学意义：为了更好地理解种子萌发这一复杂的发育过程，我们对草菇种子进行了蛋白质组分析。草籽本研究首次对枸杞种子层积过程中的蛋白质组学和微结构进行了全面的分析。西氏菌在80种蛋白质中，共鉴定出26种蛋白质，其中7种和14种蛋白质含量明显增加或减少。鉴定出的许多关键蛋白质参与了胚发育、淀粉合成和能量代谢，层积种子的显微结构分析表明，淀粉降解是种胚生长的准备过程。这些数据有助于我们对M.天女兰种子萌发(C)2015 Elsevier B. V.版权所有。
Magnolia sieboldii is a deciduous tree native to China. This species has a deep dormancy characteristic. To better understand seed germination, we used protein analysis of changes in seed protein at 0, 65, 110 and 150 d of stratification. Comparative 2DE analysis of M. sieboldii seed protein profiles at 0, 65,110 and 150 d of stratification revealed 80 differentially abundance protein species. Comparative analysis showed that ADP-glucose pyrophosphorylase small subunit was degraded during germination. In particular, it was degraded almost completely at 110 d of germination. Starch granules in the microstructure decreased after 65 d of stratification. Starch granules provided a sufficient amount of substrates and ATPs for subsequent germination. Four storage protein species were identified, of which all were down accumulated. Spots 44 and 46 had different MW and pi values, spots 36 and 46 had nearly the same MW with pI shift in the 2-DE gels, suggesting that they might be present as different isoforms of the same protein family and the post translational modification. Our results suggested that degradation of starch granules and storage protein species prepared the seed embryo for growth, as well as regulated seed germination. The present proteomics analysis provides novel insights into the mobilisation of nutrient reserves during the germination of M. sieboldii seeds.Biological significance: To better understand seed germination, a complex developmental process, we developed a proteome analysis of M. sieboldii seed. We performed the first comprehensive proteomic and microstructure analysis during different seed stratification stages of M. sieboldii. Among the 80 protein species, 26 were identified, 7 and 14 protein species were up or down accumulated significantly. Many of the identified key proteins were involved in embryo development starch biosynthesis and energy metabolism, Microstructure of stratification seed analysis revealed degradation of starch was used for preparing the seed embryo for growth. These data may help us to develop a comprehensive understanding of the physiological status and mobilisation mechanisms in M. sieboldii seed germination. (C) 2015 Elsevier B.V. All rights reserved.