Redox regulatory and anti-apoptotic functions of thioredoxin depend on S-nitrosylation at cysteine 69
Redox regulatory and anti-apoptotic functions of thioredoxin depend on S-nitrosylation at cysteine 69
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DOI：
10.1038/ncb851
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发表时间：
2002-10-01
影响因子：
21.3
通讯作者：
Dimmeler, S
中科院分区：
文献类型：
--
作者：
Haendeler, J;Hoffmann, J;Dimmeler, S
硫氧还蛋白1(Trx)是一种已知的氧化还原调节因子，参与氧化还原控制细胞生长和抑制细胞凋亡。在这里，我们表明Trx对于维持内皮细胞中S-硝基分子的含量是必不可少的。Trx本身在碱性条件下是半胱氨酸69位的S亚硝化反应，这种S亚硝化反应是清除活性氧和保持Trx氧化还原调节活性所必需的。Trx的S亚硝化也有助于Trx的抗细胞凋亡作用。因此，只有当半胱氨酸69被S硝化时，Trx才能在内皮细胞中发挥其完整的氧化还原调节和抗凋亡功能。
Thioredoxin 1 (Trx) is a known redox regulator that is implicated in the redox control of cell growth and apoptosis inhibition. Here we show that Trx is essential for maintaining the content of S-nitrosylated molecules in endothelial cells. Trx itself is S-nitrosylated at cysteine 69 under basal conditions, and this S-nitrosylation is required for scavenging reactive oxygen species and for preserving the redox regulatory activity of Trx. S-nitrosylation of Trx also contributes to the anti-apoptotic function of Trx. Thus, Trx can exert its complete redox regulatory and anti-apoptotic functions in endothelial cells only when cysteine 69 is S-nitrosylated.