A new rapid method for quantitating radioactive proline, 4-hydroxyproline, and 3-hydroxyproline.
A new rapid method for quantitating radioactive proline, 4-hydroxyproline, and 3-hydroxyproline.
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一种快速定量放射性脯氨酸、4-羟脯氨酸和 3-羟脯氨酸的新方法。
DOI：
10.1016/0003-2697(80)90155-4
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发表时间：
1980
影响因子：
2.9
通讯作者：
Nitecki,DE
中科院分区：
文献类型：
--
作者：
Tseng,SC;Stern,R;Nitecki,DE
建立了一种简便、快速的高压电泳分离脯氨酸、4-羟脯氨酸和3-羟脯氨酸的方法。一个很好的计数效率的脯氨酸实现了一个简单的提取标记材料从纸上，计数率的回收率为98-100%，相当于从柱色谱回收。该方法可以耐受样品中高浓度的盐、酸和蛋白质。不需要载体，多个样品（最多15个）可以在一张纸上分离，并在2小时内进行分析。连续稀释实验显示出良好的线性。在较宽的放射性范围内，样品的平均回收率为92%，证明了该方法的高灵敏度。该分析适用于蛋白质水解产物和在蛋白质存在下测定游离氨基酸。因此，脯氨酸、4-羟基脯氨酸和3-羟基脯氨酸可以在任何生物样品中同时定量。
A simple and rapid method for separating proline, 4-hydroxyproline and 3-hydroxyproline was developed by the use of high-voltage electrophoresis. An excellent counting efficiency of proline was achieved by a simple extraction of the labeled material from paper; 98–100% recovery of count rates was obtained, equivalent to recoveries from column chromatography. This method can tolerate high concentrations of salt, acid, and protein in the sample. No carrier is required and multiple samples (up to 15) can be separated on a single sheet and analyzed within 2 h. Serial dilution experiments showed excellent linearity. An average recovery rate of 92% was obtained for samples over a wide range of radioactivity and high sensitivity of the method was demonstrated. This analysis is applicable to protein hydrolysates and to determination of the free amino acids in the presence of protein. Thus, proline, 4-hydroxyproline and 3-hydroxyproline can be quantitated simultaneously in any biological sample.