Macromolecular neutron crystallography at the Protein Crystallography Station (PCS).
Macromolecular neutron crystallography at the Protein Crystallography Station (PCS).
复制标题
蛋白质晶体学站 (PCS) 的大分子中子晶体学。
DOI：
10.1107/s0907444910027198
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Langan,Paul
中科院分区：
文献类型：
--
作者：
Kovalevsky,Andrey;Fisher,Zoe;Johnson,Hannah;Mustyakimov,Marat;Waltman,MaryJo;Langan,Paul
洛斯阿拉莫斯中子科学中心的蛋白质晶体学站 (PCS) 是一条高性能束线，构成中子大分子结构和功能测定能力的核心。中子衍射是一种用于定位氢原子的强大技术，因此可以提供有关生物大分子如何发挥作用以及如何与彼此和较小分子相互作用的独特信息。 PCS 的用户可以使用中子束时间、氘化设施、蛋白质表达和稳定同位素底物合成，还支持数据简化和结构分析。该束线利用散裂中子的脉冲性质和大型电子探测器，以便使用白束中所有可用的中子收集波长分辨的劳厄图案。描述了 PCS 用户设施并介绍了用户程序的亮点。
The Protein Crystallography Station (PCS) at Los Alamos Neutron Science Center is a high-performance beamline that forms the core of a capability for neutron macromolecular structure and function determination. Neutron diffraction is a powerful technique for locating H atoms and can therefore provide unique information about how biological macromolecules function and interact with each other and smaller molecules. Users of the PCS have access to neutron beam time, deuteration facilities, the expression of proteins and the synthesis of substrates with stable isotopes and also support for data reduction and structure analysis. The beamline exploits the pulsed nature of spallation neutrons and a large electronic detector in order to collect wavelength-resolved Laue patterns using all available neutrons in the white beam. The PCS user facility is described and highlights from the user program are presented.