Relationship Between Total Tear IgE and Specific Serum IgE in Autumnal Allergic Conjunctivitis
Relationship Between Total Tear IgE and Specific Serum IgE in Autumnal Allergic Conjunctivitis
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DOI：
10.1097/ico.0b013e318253214b
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发表时间：
2013-01-01
期刊：
影响因子：
2.8
通讯作者：
Amano, Shiro
中科院分区：
文献类型：
--
作者：
Mimura, Tatsuya;Usui, Tomohiko;Amano, Shiro
目的：最近开发了一种用于测定总免疫球蛋白E（IgE）的新的商业免疫层析试验（Allerwatch）。我们以前曾报道过使用该试剂盒分析在春季收集的泪液样本。在这项研究中，我们研究了秋季过敏性结膜炎患者泪液中总IgE水平与血清特异性IgE水平之间的关系。方法：对36例秋季过敏性结膜炎患者进行了前瞻性、非随机、横断面研究32例年龄、性别相匹配的健康对照者（对照组）和10例流行性角结膜炎患者。使用Allerwatch测试确定所有受试者的总泪液IgE评分（0、1和2），而12种吸入性过敏原的血清总IgE和特异性IgE水平分别使用Phadezym PRIST和CAP-RAST系统测量。与对照组和流行性角结膜炎组相比，秋季过敏组的总泪液IgE阳性率显著较高（88.9% vs. 0.0% vs. 0.0%，P < 0.00001）。在秋季过敏组中，泪液总IgE评分与对数转换的血清总IgE水平之间存在显著相关性（r = 0.84）。与屋尘螨（r = 0.53）、屋尘（r = 0.51）、螨类（r = 0.49）、雪松花粉（r = 0.33）和艾蒿（r = 0.33）的血清IgE水平也有显著相关性。多因素Logistic回归分析显示，泪液总IgE评分的唯一显著预测因子是对数转换的血清总IgE水平（比值比= 2.76，P < 0.00001）。Allerwatch总泪液IgE评分与抗屋尘螨特异性IgE血清水平之间的相关性表明，屋尘螨过敏原可能是过敏性结膜炎的主要原因，日本的秋天Allerwatch方法可以提供泪液中总IgE的快速测量，这使得在门诊环境中容易地诊断过敏性结膜炎成为可能。
Purpose: A new commercial immunochromatographic test (Allerwatch) has recently been developed for determining total immunoglobulin E (IgE). We previously reported on the use of this kit for analyzing tear fluid samples collected during the spring. In this study, we examined the relationship between the total IgE level in tears and the specific serum IgE levels in patients with autumnal allergic conjunctivitis.Methods: A prospective, nonrandomized cross-sectional study was conducted in 36 patients with autumnal allergic conjunctivitis (autumnal allergic group), in 32 age-matched and sex-matched healthy control subjects (control group), and in 10 patients with epidemic keratoconjunctivitis. Total tear IgE score was determined in all subjects using the Allerwatch test (0, 1, and 2), whereas the serum levels of total IgE and specific IgE for 12 inhaled allergens were measured using the Phadezym PRIST and CAP-RAST systems, respectively.Results: Positivity rates for total tear IgE were significantly higher in the autumnal allergic group as compared with the control and epidemic keratoconjunctivitis groups (88.9% vs. 0.0% vs. 0.0%, P < 0.00001). In the autumnal allergic group, there was a significant correlation between the total tear IgE score and the log-transformed total serum IgE level (r = 0.84). Significant correlations were also seen with the IgE serum levels for Dermatophagoides pteronyssinus (r = 0.53), house dust (r = 0.51), acarus (r = 0.49), cedar pollen (r = 0.33), and mugwort (r = 0.33). Multivariate logistic regression analysis showed that the only significant predictor of the total tear IgE score was the log-transformed total serum IgE level (odds ratio = 2.76, P < 0.00001).Conclusions: The correlation between the Allerwatch total tear IgE score and the specific IgE serum levels against house dust mites suggests that house dust mite allergens might be the primary cause of allergic conjunctivitis during autumn in Japan. The Allerwatch methodology can provide rapid measurements of total IgE in the tear fluid, which makes it possible to easily diagnose allergic conjunctivitis in an outpatient setting.