Investigating Tissue Regeneration Using the DUAL Control Genetic Ablation System.
Investigating Tissue Regeneration Using the DUAL Control Genetic Ablation System.
复制标题
使用双控制基因消融系统研究组织再生。
DOI：
10.1007/978-1-0716-2847-8_18
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Harris,RE
中科院分区：
文献类型：
--
作者：
Harris,RE
基因消融是研究体内再生的一种高效方法，通过刺激组织特异性细胞死亡，随后诱导发育中的生物体的再生和修复。这种方法在果蝇身上尤其成功，已经开发了各种基于温度的基因消融工具来探索幼虫成像盘再生的复杂性。在这里，我们描述了最近建立的称为DUAL Control的消融系统的使用，该系统可用于表征损伤反应并通过基因操作囊胚细胞来识别新的再生调节因子。
Genetic ablation is a highly efficient method to study regeneration in vivo by stimulating tissue-specific cell death that subsequently induces regrowth and repair in a developing organism. This approach has been particularly successful inDrosophila, for which various temperature-based genetic ablation tools have been developed to explore the complexities of regeneration in larval imaginal discs. Here, we describe the use of a recently established ablation system called DUAL Control, which can be used to both characterize the damage response and genetically manipulate blastema cells to identify novel regulators of regeneration.