Noninvasive stratification of postinfarction rats based on the degree of cardiac dysfunction using magnetic resonance imaging and echocardiography
Noninvasive stratification of postinfarction rats based on the degree of cardiac dysfunction using magnetic resonance imaging and echocardiography
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DOI：
10.1152/ajpheart.00668.2016
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发表时间：
2017-05-01
影响因子：
4.8
通讯作者：
Sjaastad, Ivar
中科院分区：
文献类型：
--
作者：
Aronsen, Jan Magnus;Espe, Emil Knut Stenersen;Sjaastad, Ivar
心肌梗死（MI）大鼠模型在现代心血管研究中起着至关重要的作用，但是该模型的固有异质性代表了一个挑战。我们试图确定MI大鼠之间的亚组，并为此类亚组建立简单的无创分层方案。诱导MI六周后，使用磁共振成像（MRI）和超声心动图进行了无创检查。十二只假手术大鼠用作对照。末期末期左心室（LV）压力增加，肺部体重充当充血性心力衰竭（CHF）的指标。使用13种非侵入性和侵入性参数的聚类算法用于鉴定动物之间的不同基团。聚类分析显示了四种不同的MI后表型。两个没有拥挤，但具有不同程度的LV扩张，两个具有不同程度的拥塞和右心（RV）的感情。在MRI参数中，RV质量作为CHF具有100％特异性/灵敏度的CHF的鲁棒性无创标记。此外，LV梗塞的大小和RV射血分数进一步预测了具有出色特异性/敏感性的非CHF和CHF大鼠之间的亚组。超声心动图参数的剩余心房直径预测的CHF。此外，LV末端直径预测了非CHF大鼠之间的亚组。我们提出了两个简单的无创方案，以根据心力衰竭程度对MI大鼠进行分层。一个用于MRI，一个用于超声心动图。新闻和值得注意的大鼠体内表型对于健壮且可靠的数据至关重要。在这里，我们提出了两个简单的非侵入性方案，用于基于心力衰竭程度：一种使用磁共振成像，一种基于超声心动图的分层。
The myocardial infarction (MI) rat model plays a crucial role in modern cardiovascular research, but the inherent heterogeneity of this model represents a challenge. We sought to identify subgroups among the post-MI rats and establish simple noninvasive stratification protocols for such subgroups. Six weeks after induction of MI, 49 rats underwent noninvasive examinations using magnetic resonance imaging (MRI) and echocardiography. Twelve sham-operated rats served as controls. Increased end-diastolic left ventricular (LV) pressure and lung weight served as indicators for congestive heart failure (CHF). A clustering algorithm using 13 noninvasive and invasive parameters was used to identify distinct groups among the animals. The cluster analysis revealed four distinct post-MI phenotypes; two without congestion but with different degree of LV dilatation, and two with different degree of congestion and right ventricular (RV) affection. Among the MRI parameters, RV mass emerged as robust noninvasive marker of CHF with 100% specificity/sensitivity. Moreover, LV infarct size and RV ejection fraction further predicted subgroup among the non-CHF and CHF rats with excellent specificity/sensitivity. Of the echocardiography parameters, left atrial diameter predicted CHF. Moreover, LV end-diastolic diameter predicted the subgroups among the non-CHF rats. We propose two simple noninvasive schemes to stratify post-MI rats, based on the degree of heart failure; one for MRI and one for echocardiography.NEW & NOTEWORTHY In vivo phenotyping of rats is essential for robust and reliable data. Here, we present two simple noninvasive schemes for the stratification of postinfarction rats based on the degree of heart failure: one using magnetic resonance imaging and one based on echocardiography.