Influence of exercise and joint topography on depth-related spatial distribution of proteoglycan and collagen content in immature equine articular cartilage
Influence of exercise and joint topography on depth-related spatial distribution of proteoglycan and collagen content in immature equine articular cartilage
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DOI：
10.2746/042516409x424162
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发表时间：
2009-07-01
影响因子：
2.2
通讯作者：
Hyttinen, M. M.
中科院分区：
文献类型：
--
作者：
Brama, P. A. J.;Holopainen, J.;Hyttinen, M. M.
进行研究的原因：有充分的证据表明关节软骨的主要生化成分在关节表面的地形异质性和负荷对其的影响，但没有关于马关节软骨蛋白多糖（PG）和胶原含量的深度相关性区域变化的信息。两种技术（番红-O密度测定法和傅里叶变换红外光谱法）应用于18个月大纯种马掌指关节近端指骨的关节软骨切片，这些纯种马从0- 10岁在牧场饲养。18个月无（PASTEX）和（CONDEX）额外运动。调查了两个研究中心：结果：关节软骨蛋白多糖含量由表层向深层逐渐增加，胶原含量由表层向深层逐渐增加，关节软骨蛋白多糖含量由表层向深层逐渐增加，胶原含量由表层向深层逐渐增加。在没有运动影响的情况下，PASTEX和CONDEX动物中部位2的PG含量显著较高。在PASTEX动物胶原蛋白含量显着较高的网站1，但在CONDEX组的情况是相反的，由于一个显着的运动效果网站1，导致胶原蛋白含量减少。结论：胶原蛋白和PG含量梯度同意在其他物种的研究结果。PG水平的观察结果表明，运动水平并不剧烈。PASTEX组的胶原结果证实了早期的研究结果，CONDEX组中I部位的胶原水平较低可能是由于胶原重塑的生理成熟过程的进展：本研究证实了早期的观察结果，即即使是早期运动水平的适度变化也可能对关节软骨的胶原网络产生显着影响。
Reasons for performing study: There is ample evidence on topographical heterogeneity of the principal biochemical components of articular cartilage over the surface of the joint and the influence of loading thereon, but no information on depth-related zonal variation in horses.Objectives: To study depth-related zonal variation in proteoglycan (PG) and collagen content in equine articular cartilage.Methods: Two techniques (safranin-O densitometry and Fourier transform infrared spectroscopy) were applied to sections of articular cartilage from the proximal phalangeal hone of the metacarpophalangeal joint of 18-month-old Thoroughbreds that had been raised at pasture from age 0-18 months without (PASTEX) and with (CONDEX) additional exercise. Two sites were investigated: site I at the joint margin that is unloaded at rest or at slow gaits, but subjected to high-intensity loading during athletic activity; and site 2, a continuously, but less intensively, loaded site in the centre of the joint.Results: Proteoglycan values increased from the surface to the deep layers of the cartilage,, collagen content showed a reverse pattern. PG content was significantly higher at site 2 in both PASTEX and CONDEX animals without an effect of exercise. In the PASTEX animals collagen content was significantly higher at site 1, but in the CONDEX group the situation was reversed, due to a significant exercise effect on site 1, leading to a reduced collagen content.Conclusions: Collagen and PG content gradients agree with findings in other species. The observations on PG levels suggest that the exercise level was not strenuous. The collagen results in the PASTEX group confirmed earlier findings, the lower levels at site I in the CONDEX group being possibly due to an advancement of the physiological maturation process of collagen remodelling.Potential relevance: This study confirms earlier observations that even moderate variations in exercise level in early age may have significant effects on the collagen network of articular cartilage.