Using a porous silicon photonic crystal for bacterial cell-based biosensing
Using a porous silicon photonic crystal for bacterial cell-based biosensing
复制标题
DOI：
10.1002/pssa.200674379
复制
发表时间：
2007-05-01
影响因子：
2
通讯作者：
Sailor, Michael J.
中科院分区：
文献类型：
--
作者：
Alvarez, Sara D.;Schwartz, Michael P.;Sailor, Michael J.
这项工作展示了一种使用一维多孔硅光子晶体和与 CCD 光谱仪耦合的白光源非侵入性监测丁香假单胞菌细菌生长和 06 病毒感染的方法。多孔硅上的细菌生长会导致散射效率增加，可以通过一维多孔硅光子晶体反射光强度的变化来测量散射效率。观察到细菌浓度与光子共振光强度之间的线性关系，检测限与光密度测量获得的检测限相似。病毒感染后，有时会观察到散射光强度的降低，这与病毒复制和随后细菌细胞破裂引起的细胞裂解有关。结果表明，测量可以在培养箱中实时进行，细胞在整个实验过程中保持在理想的环境中。这种方法非常适合基于细胞的生物传感，因为可以远程监测细菌细胞，而不需要对细胞进行电镀或光密度测量采样。 (c) 2007 年 WILEY-VCH Verlag GmbH & Co. KGaA，魏因海姆。
This work demonstrates a method for non-invasively monitoring the growth and 06 virus infection of Pseudomonas syringae bacteria using a 1-D porous silicon photonic crystal and a white-light source coupled to a CCD spectrometer. Bacteria growth on porous Si leads to increased scattering efficiency that can be measured as a change in intensity of light reflected from a 1-D porous Si photonic crystal. A linear relationship between bacteria concentration and intensity of light at the photonic resonance is observed, and detection limits are similar to those obtained from optical density measurements. Upon infection with virus, decreases in scattered light intensity are observed at times that correlate with cell lysis caused by viral replication and subsequent bursting of bacteria cells. It is shown that measurements can be performed in real-time and in an incubator where cells remain in their ideal environment throughout the experiment. This method is well-suited for cell-based biosensing, because bacteria cells can be monitored remotely without the need for sampling cells for plating or optical density measurements. (c) 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.