Immunohistochemical examination using the pericyte marker myosin 1B in a perivascular myoid tumor of soft tissue with definitive pericytic differentiation.
Immunohistochemical examination using the pericyte marker myosin 1B in a perivascular myoid tumor of soft tissue with definitive pericytic differentiation.
复制标题
使用周细胞标记物肌球蛋白 1B 对具有明确周细胞分化的软组织血管周围肌样瘤进行免疫组织化学检查。
DOI：
10.1111/pin.12777
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Iwashita T.
中科院分区：
文献类型：
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作者：
Meguro S;Matsushima S;Enomoto Y;Kawasaki H;Kosugi I;Tsuchida T;Baba S;Fukamizu H;Yamato Y;Iwashita T.
致编辑：在此，我们报告了一个软组织肿瘤的情况下，从远端肢体，被证明是由肿瘤细胞与明确的周细胞分化，通过免疫组化分析与周细胞标记肌球蛋白1B（MYO 1B）。1在我们之前的研究中，我们确定MYO 1B是一种新的周细胞标记物，在周细胞中表达，但在血管平滑肌细胞（VSMC）中不表达。基于MYO 1B和高分子量钙调素（hCD; SMC的特异性标志物）的表达模式，将血管壁细胞分为3种类型的细胞，平滑肌肌动蛋白（aSMA）阳性（α SMA）/MYO 1B（α SMA）/hCD阴性（α SMA）周细胞、aSMA（α SMA）MYO 1B（α MYO）hCD（α MYO）VSMC和具有中间特征的aSMA（α SMA）MYO 1B（α MYO）hCD（α MYO）血管壁细胞。然后，我们将这种血管壁细胞分类应用于血管周围肌样肿瘤（血管球瘤和肌外皮细胞瘤），发现具有周细胞特征的aSMA（SMA）MYO 1B（MYO 1B）hCD（hCD）肿瘤细胞仅见于血管球瘤，而非肌外皮细胞瘤。然而，在24例血管球瘤病例中，具有周细胞特征的aSMA（SMA）MYO 1B（MYO 1B）hCD（MYO 1B）肿瘤细胞的比例范围为0%至40%。此外，我们以前从未遇到过完全由明确的细胞周分化的肿瘤细胞组成的血管周围肌样肿瘤。一位69岁的日本男性10年前在他的右前臂发现了一个肿瘤结节，从那时起肿瘤逐渐增大。未发现肿瘤的特异性临床体征或症状。他住进了滨松大学医院，之后肿瘤（位于表皮下）被诊断为表皮囊肿并切除。大体尺寸为30 × 20 × 20 mm，肿瘤被包裹，边界清楚，中心坏死（图1a）。显微镜下，肿瘤中央部分坏死（图S1 a）。在结节的周边部分存在具有分支小血管的活的椭圆形肿瘤细胞（图1b，c）。此外，还观察到扩张的海绵状间隙。有丝分裂罕见，在10个高倍镜（400）视野中不超过1个细胞进行有丝分裂，表明该肿瘤
To the Editor: Herein, we report a soft tissue tumor case arising from a distal extremity that was shown to be composed of tumor cells with definitive pericytic differentiation through immunohistochemical analysis with the pericyte marker myosin 1B (MYO1B). 1 In our previous study, we identified MYO1B as a new pericyte marker that is expressed in pericytes but not vascular smooth muscle cells (VSMCs). Based on the expression pattern of MYO1B and high molecular weight caldesmon (hCD; a specific marker for SMCs), vascular mural cells were classified into three types of cells, asmooth muscle actin (aSMA)-positive (þ)/MYO1B (þ)/hCD-negative(À) pericytes, aSMA (þ) MYO1B (À) hCD (þ) VSMCs, and aSMA (þ) MYO1B (þ) hCD (þ) vascular mural cells with intermediate features. We then applied this vascular mural cell classification for perivascular myoid tumors (glomus tumor and myopericytoma) and discovered that aSMA (þ) MYO1B (þ) hCD (À) tumor cells with pericytic features were only found in glomus tumors but not myopericytomas. However, the proportion of aSMA (þ) MYO1B (þ) hCD (À) tumor cells with pericytic features in 24 glomus tumor cases ranged from 0% to 40%. Furthermore, we have not previously encountered perivascular myoid tumors that were entirely composed of tumor cells with definitive pericytic differentiation.A 69-year-old Japanese male noticed a tumor nodule on his right forearm 10 years ago that has since then gradually increased in size. No specific clinical signs or symptoms of the tumor had been noted. He was admitted to Hamamatsu University Hospital, after which the tumor (located in the subepidermis) was diagnosed as an epidermal cyst and resected. In its gross dimensions, the lesion was a 30 Â20 Â20mm encapsulated and well-circumscribed tumor with necrosis at its center (Fig. 1a). Microscopically, the central part of the tumor was necrotic (Fig. S1a). The living ovalshaped tumor cells with branching smaller vessels were present at the peripheral part of the nodule (Fig. 1b, c). In addition, dilated cavernous spaces were observed. Mitosis was rare, with no more than one cell undergoing mitosis in 10 high-powered (400Â) fields, indicating that this tumor