Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients
Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients
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DOI：
10.1016/j.jchromb.2007.03.003
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发表时间：
2007-06-15
影响因子：
3
通讯作者：
Nishimura, Shin-Ichiro
中科院分区：
文献类型：
--
作者：
Nakagawa, Hiroaki;Hato, Megumi;Nishimura, Shin-Ichiro
改变的N-糖基化发生在许多疾病中。例如，在类风湿性关节炎（RA）中，已经观察到IgG中半乳糖残基的减少和α 1-酸性糖蛋白中岩藻糖残基的增加。为了进一步分析疾病中的N-聚糖，我们采用反相高效液相色谱/负离子模式，通过声喷雾电离离子阱质谱法和吡啶胺化，显示了来自全血清的N-聚糖谱。比较15例女性RA患者和18例年龄匹配的健康女性的资料。在RA中观察到的最显著变化是单半乳糖基双触角N-聚糖水平降低，与先前关于IgG的报告一致。我们还显示了以前未报道的异构体之间的差异和增加的三触角寡糖。这些结果表明，全血清N-聚糖的LC-MS分析可以识别RA中的N-聚糖改变，这是一种有前途的方法，用于RA机制的研究和诊断。(c)2007 Elsevier B. V.保留所有权利。
Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in alpha 1-acid glycoprotein have been observed. To further analyse N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with the previous reports regarding IgG. We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC-MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis. (c) 2007 Elsevier B.V. All rights reserved.