Converging effects of a Bifidobacterium and Lactobacillus probiotic strain on mouse intestinal physiology
Converging effects of a Bifidobacterium and Lactobacillus probiotic strain on mouse intestinal physiology
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DOI：
10.1152/ajpgi.00401.2013
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发表时间：
2014-07-15
影响因子：
4.5
通讯作者：
Hyland, Niall P.
中科院分区：
文献类型：
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作者：
Lomasney, Kevin W.;Cryan, John F.;Hyland, Niall P.
越来越多的证据支持益生菌在治疗胃肠道疾病方面的功效，其中许多疾病与液体和电解质运输失调有关。现在越来越多的证据表明，宿主微生物群和益生菌可以影响肠道离子转运，并且这些影响通常以菌株依赖性方式发生。在本研究中，我们试图研究两种治疗相关微生物（婴儿双歧杆菌 35624 和唾液乳杆菌 UCC118）对小肠转运、粪便排出量和水含量、跨上皮阻力 (TER) 和结肠分泌运动功能的影响。喂食任一菌株的小鼠均表现出体内小肠转运显着减少，但任一菌株均不影响粪便颗粒排出量或水含量。喂食这两种生物的小鼠的结肠显示结肠 TER 增加，但紧密连接蛋白 claudin 1 和 occludin 的基因表达没有伴随变化。然而，唾液乳杆菌 UCC118 选择性抑制饲喂这种特殊益生菌的动物组织中神经诱发的离子分泌。与这一发现一致的是，神经毒素河豚毒素 (TTx) 在加入尤斯室的结肠制剂后显着抑制了唾液乳杆菌 UCC118 诱导的短路电流反应。对婴儿双歧杆菌 35624 的反应也表现出对 TTx 的敏感性，尽管程度明显低于唾液乳杆菌 UCC118。两种菌株在添加到尤斯室后同样抑制胆碱能诱导的离子转运。总而言之，这些数据表明婴儿双歧杆菌 35624 和唾液乳杆菌 UCC118 可能适用于与小肠运输增加相关的疾病，特别是唾液乳杆菌 UCC118，神经介导的腹泻。
Evidence has grown to support the efficacy of probiotics in the management of gastrointestinal disorders, many of which are associated with dysregulated fluid and electrolyte transport. A growing body of evidence now suggests that the host microbiota and probiotics can influence intestinal ion transport and that these effects often occur in a strain-dependent manner. In this study, we sought to investigate the effects of two therapeutically relevant organisms, Bifidobacterium infantis 35624 and Lactobacillus salivarius UCC118, on small intestinal transit, fecal output and water content, transepithelial resistance (TER), and colonic secretomotor function. Mice fed either strain displayed significantly reduced small intestinal transit in vivo, though neither strain influenced fecal pellet output or water content. Colon from mice fed both organisms displayed increased colonic TER, without a concomitant change in the gene expression of the tight junction proteins claudin 1 and occludin. However, L. salivarius UCC118 selectively inhibited neurally evoked ion secretion in tissues from animals fed this particular probiotic. Consistent with this finding, the neurotoxin tetrodotoxin (TTx) significantly inhibited the short-circuit current response induced by L. salivarius UCC118 following addition to colonic preparations in Ussing chambers. Responses to B. infantis 35624 also displayed sensitivity to TTx, although to a significantly lesser degree than L. salivarius UCC118. Both strains similarly inhibited cholinergic-induced ion transport after addition to Ussing chambers. Taken together, these data suggest that B. infantis 35624 and L. salivarius UCC118 may be indicated in disorders associated with increased small intestinal transit, and, in particular for L. salivarius UCC118, neurally mediated diarrhea.