Simultaneous expression of glutathione, thioredoxin-1, and their reductases in nerve transected hypoglossal motor neurons of rat
Simultaneous expression of glutathione, thioredoxin-1, and their reductases in nerve transected hypoglossal motor neurons of rat
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DOI：
10.1016/j.brainres.2009.10.014
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发表时间：
2010-01-08
期刊：
影响因子：
2.9
通讯作者：
Kiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Hama, Isuzu;Nakagomi, Saya;Kiyama, Hiroshi
抗氧化应激反应对于神经损伤的运动神经元的存活至关重要。在此，我们研究了谷胱甘肽还原酶（GSHr），硫氧还蛋白（TRX 1和TRX 2），硫氧还蛋白还原酶（TRXr 1和TRXr 2），抗氧化途径的重要组成部分，大鼠舌下神经横断后的表达变化。RT-PCR和原位杂交表明，GSHr，TRX 1和TRXr 1的mRNA在神经损伤的运动神经元的前几周内显着上调，而TRX 2和TRXr 2的mRNA在整个8周神经横断后不变。免疫组织化学分析证实了GSH，GSHr，TRX 1和TRXr 1蛋白在损伤神经元中的上调。Western blotting也证实了GSHr、TRX 1和TRXr 1在损伤神经元中的上调。这些数据表明，两个主要的氧化还原系统，GSH/GSHr和TRX 1/TRXr 1，在受损的神经元中同时被激活，并可能提供保护受损的神经元免受氧化应激。(C)2009 Elsevier B. V.保留所有权利。
Anti-oxidative stress responses are crucial for the survival of nerve-injured motor neurons. Herein, we examined changes in expression of glutathione reductase (GSHr), thioredoxins (TRX1 and TRX2), and thioredoxin reductases (TRXr1 and TRXr2), important constituents of anti-oxidative pathways, following rat hypoglossal nerve transection. RT-PCR and in situ hybridization demonstrated that GSHr, TRX1, and TRXr1 mRNAs were significantly upregulated during the first few weeks in nerve-injured motor neurons, while TRX2 and TRXr2 mRNAs were unchanged throughout 8 weeks after nerve transection. The up-regulation of GSH, GSHr, TRX1, and TRXr1 proteins in injured neurons was confirmed by immunohistochemical analysis. Western blotting also demonstrated up-regulation of GSHr, TRX1, and TRXr1 in injured neurons. These data suggest that the two major redox systems, GSH/GSHr and TRX1/TRXr1, are simultaneously activated in injured neurons, and likely provide protection of injured neurons against oxidative stress. (C) 2009 Elsevier B.V. All rights reserved.