Assessment of proliferating cell nuclear antigen activity using digital image analysis in breast carcinoma following magnetic resonance-guided interstitial laser photocoagulation.
Assessment of proliferating cell nuclear antigen activity using digital image analysis in breast carcinoma following magnetic resonance-guided interstitial laser photocoagulation.
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DOI：
10.1046/j.1524-4741.2003.09509.x
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发表时间：
2003-09-01
期刊：
影响因子：
--
通讯作者：
Harms, Steven
中科院分区：
文献类型：
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作者：
Korourian, Soheila;Klimberg, Suzanne;Harms, Steven
本研究检测了经组织学证实的浸润性乳腺癌患者接受磁共振引导的间质激光光凝（MR-GILP）治疗后肿瘤细胞的增殖活性。使用增殖细胞核抗原（PCNA）的免疫组织化学标记物，PCNA是活跃增殖细胞中丰富的核蛋白。该研究证明了MR-GILP在消融浸润性乳腺癌肿瘤细胞方面的有效性。诊断为浸润性乳腺癌证实了核心针活检。使用专门设计的磁共振成像（MRI）设备，旋转输送激发非共振（牛仔竞技表演），测量肿瘤的最大尺寸范围为1.8至4.0 cm。分析了来自7名浸润性癌患者的7份福尔马林固定、石蜡包埋的存档组织，这些组织处于MR-GILP后状态。使用PCNA免疫过氧化物酶（Biomeda公司），测定激光光凝焦点周围剩余肿瘤细胞的增殖能力。使用带有自动载物台的Nikon Eclipse E800显微镜数字化采集病变。使用Cool SNAP图像编辑软件（版本1.0）分析图像。为阳性染色设置适当的阈值，并以距离激光消融中心的径向毫米间隔比较所有样品之间相等面积的有限同心径向测量值。从木炭部位开始，每毫米径向上PCNA阳性细胞所占的积分面积（激光中心）随着距离该位置的增加而增加（每个径向测量的平均值显示：在1 mm半径处，正积分面积为0.0024 mm 2;在2 mm处，为0.0145 mm 2;在3 mm处，为0.0351 mm 2;在4 mm处，0.0696mm2;在5 mm处，0.1025mm2;以及在6 mm处，0.1263mm2）。MR-GILP是一种有效的消融乳腺癌的手段。这种治疗选择可能代表了对于单个病灶≤ 1 cm的乳房肿瘤切除术的替代方案，或者使具有两个单独病灶的患者适合乳房肿瘤切除术。
This study examines proliferative activity in tumor cells of patients with histologically documented invasive breast carcinoma treated with magnetic resonance-guided interstitial laser photocoagulation (MR-GILP). Immunohistochemical marker for proliferating cell nuclear antigen (PCNA), a nuclear protein abundant in actively proliferating cells, is used. The study demonstrates the effectiveness of MR-GILP in ablating tumor cells of infiltrating breast cancer. The diagnosis of infiltrating breast carcinoma was confirmed by core needle biopsies. Using a specially designed magnetic resonance imaging (MRI) device, rotating delivery of excitation off-resonance (RODEO), tumors were measured ranging from 1.8 to 4.0 cm in greatest dimension. Seven formalin-fixed, paraffin-embedded archival tissues from seven patients with infiltrating carcinoma, status post-MR-GILP, were analyzed. Using PCNA immunoperoxidase (Biomeda Corp.), the proliferative capability of the remaining tumor cells around the focus of laser photocoagulation was determined. The lesions were digitally acquired using a Nikon Eclipse E800 microscope with an automated stage. Images were analyzed using Cool SNAP image editing software (version 1.0). Appropriate thresholds were set for positive staining and limited concentric radial measurements of equal area between all samples were compared at radial millimeter intervals from the center of laser ablation. The integrated area occupied by PCNA-positive cells per radial millimeter from the charcoal site (the center of the laser) increased as the distance from this site increased (a mean average at each radial measurement revealed: at the 1 mm radius the positive integrated area was 0.0024 mm2; at 2 mm, 0.0145 mm2; at 3 mm, 0.0351 mm2; at 4 mm, 0.0696 mm2; at 5 mm, 0.1025 mm2; and at 6 mm, 0.1263 mm2). MR-GILP is an effective mean of ablating breast carcinoma. This treatment option may represent an alternative to lumpectomy for a single lesion < or =1 cm, or make patients with two separate lesions eligible for lumpectomy.