Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellular Matrix.
Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellular Matrix.
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DOI：
10.1007/978-1-4939-9095-5_12
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发表时间：
2019
影响因子：
--
通讯作者：
U. Hansen
中科院分区：
文献类型：
--
作者：
U. Hansen
整合素介导的细胞与细胞外基质（ECM）的相互作用对它们的活性很重要。ECM本身是一个复杂的大分子网络，形成聚集体或上层结构。此外，分子组成对ECM的大分子组织和功能性质也很重要。此外，胶原蛋白分子在掺入原纤维后失去整合素结合能力。因此，我们建立了在超分子水平上分析整合素-矩阵相互作用的详细协议。
Integrin-mediated interactions of cells with the extracellular matrix (ECM) are important for their activities. The ECM itself is a complex network of macromolecules forming aggregates or suprastructures. Moreover, the molecular composition is important for the macromolecular organization and, thereby, the functional properties of the ECM. In addition, collagen molecules lose their integrin-binding capabilities after incorporation into fibrils. Therefore, we have established detailed protocols for the analysis of integrin-matrix interactions at the supramolecular level.