Genome-wide identification, functional and evolutionary analysis of terpene synthases in pineapple
Genome-wide identification, functional and evolutionary analysis of terpene synthases in pineapple
复制标题
菠萝萜烯合酶的全基因组鉴定、功能和进化分析
DOI：
10.1016/j.compbiolchem.2017.05.010
复制
发表时间：
2017-10-01
影响因子：
3.1
通讯作者：
Li, Guanglin
中科院分区：
文献类型：
--
作者：
Chen, Xiaoe;Yang, Wei;Li, Guanglin
萜类合成酶是生物合成活性萜类化合物的重要酶类，具有重要的生理、生态和药用价值。虽然菠萝（Ananas comosus）中有萜类化合物的报道，但对菠萝萜类化合物合成的TPS基因的全基因组研究仍然缺乏。通过整合菠萝基因组和蛋白质组学数据，在菠萝中发现21个推测的萜烯合成酶基因，并将其划分为5个亚家族。串联重复是TPS基因家族重复的原因。此外，每个TPS亚家族之间的功能分化可能有以下几个原因。在TPS-a和TPS-c亚家族之间鉴定出62个关键氨基酸位点存在ii型功能差异。最后，共同进化分析表明多个氨基酸残基参与了共同进化过程。此外，还检测了两种tps的酶活性。通过对菠萝TPS基因的全基因组鉴定、功能和进化分析，为了解TPS家族的作用提供了新的视角，并为进一步表征TPS基因家族的功能和进化奠定了基础。(C) 2017 Elsevier Ltd.版权所有。
Terpene synthases (TPSs) are vital for the biosynthesis of active terpenoids, which have important physiological, ecological and medicinal value. Although terpenoids have been reported in pineapple (Ananas comosus), genome-wide investigations of the TPS genes responsible for pineapple terpenoid synthesis are still lacking. By integrating pineapple genome and proteome data, twenty-one putative terpene synthase genes were found in pineapple and divided into five subfamilies. Tandem duplication is the cause of TPS gene family duplication. Furthermore, functional differentiation between each TPS subfamily may have occurred for several reasons. Sixty-two key amino acid sites were identified as being type-II functionally divergence between TPS-a and TPS-c subfamily. Finally, coevolution analysis indicated that multiple amino acid residues are involved in coevolutionary processes. In addition, the enzyme activity of two TPSs were tested. This genome-wide identification, functional and evolutionary analysis of pineapple TPS genes provide a new insight into understanding the roles of TPS family and lay the basis for further characterizing the function and evolution of TPS gene family. (C) 2017 Elsevier Ltd. All rights reserved.