Serum autoantibodies to brain in Landau-Kleffner variant, autism, and other neurologic disorders
Serum autoantibodies to brain in Landau-Kleffner variant, autism, and other neurologic disorders
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DOI：
10.1016/s0022-3476(99)70248-9
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发表时间：
1999-05-01
影响因子：
5.1
通讯作者：
Deuel, RK
中科院分区：
文献类型：
--
作者：
Connolly, AM;Chez, MG;Deuel, RK
目的：经常有报道称，病因不明的语言和社会发展障碍在接受免疫调节方案治疗的患者中得到改善。在这里，我们确定了这些儿童中大脑自身抗体的频率。 设计：我们从一组儿童中收集了血清，这些儿童患有（I）纯兰道-克莱夫纳综合征（n = 2），（2）兰道-克莱夫纳综合征变异体（LKSV，n = 11）和（3）自闭症谱系障碍（ASD，n = 11）。在获得血清样本之前，没有人接受过免疫调节治疗，对照血清 (n = 71) 来自 29 名健康儿童、22 名患有非神经系统疾病 (NNI) 的儿童和 20 名患有其他神经系统疾病 (OND) 的儿童。我们使用市售试剂盒对人颞叶皮层和抗核自身抗体进行免疫染色，从而鉴定出脑自身抗体。结果：LKSV 儿童血清中存在 IgG 抗脑自身抗体，ASD 儿童血清中存在 27%，OND 儿童血清中存在 10%，而健康儿童和 NNI 对照儿童的血清中只有 2% 存在 IgG 抗脑自身抗体。 36% 的 ASD 儿童血清中存在 IgM 自身抗体，9% 的 LKSV 儿童血清中存在 IgM 自身抗体，15% 的 OND 儿童血清中存在 IgM 自身抗体，而对照血清中这一比例为 0%。标记研究确定了一种抗原靶标是内皮细胞。滴度大于或等于1:80的抗核抗体在ASD儿童和ONBs对照儿童中更为常见。结论:LKSV和ASD儿童比NNIs儿童或健康儿童血清中脑内皮细胞和细胞核抗体的出现频率更高。这些抗体的存在提出了一种可能性，即自身免疫在患有这些疾病的儿童的语言和社会发育异常的发病机制中发挥作用。
Objective: Etiologically unexplained disorders of language and social development hale often been reported to improve in patients treated with immune-modulating regimens. Here we determined the frequency of autoantibodies to brain among such children.Design: We collected sera from a cohort of children with (I) pure Landau-Kleffner syndrome (n = 2), (2) Landau-Kleffner syndrome variant (LKSV, n = 11), and (3) autistic spectrum disorder (ASD, n = 11). None had received immune-modulating treatment before the serum sample was obtained, Control sera (n = 71) were from 29 healthy children, 22 with nonneurologic illnesses (NNIs), and 20 children with other neurologic disorders (ONDs). We identified brain autoantibodies by immunostaining of human temporal cortex and antinuclear autoantibodies using commercially available kits.Results: IgG anti-brain autoantibodies were present in 45% of sera from children with LKSV, 27% with ASD, and 10% with ONDs compared with 2% from healthy children and control children with NNIs. IgM autoantibodies were present in 36% of sera from children with ASD, 9% with LKSV, and 15% with ONDs compared with 0% of control sera. Labeling studies identified one antigenic target to be endothelial cells. Antinuclear antibodies with titers greater than or equal to 1:80 were more common in children with ASD and control children with ONBs,Conclusion: Children with LKSV and ASD have a greater frequency of serum antibodies to brain endothelial cells and to nuclei than children with NNIs or healthy children. The presence of these antibodies raises the possibility that autoimmunity plays a role in the pathogenesis of language and social developmental abnormalities in a subset of children with these disorders.