AGING AND RESISTANCE TO OXIDATIVE DAMAGE IN CAENORHABDITIS-ELEGANS
AGING AND RESISTANCE TO OXIDATIVE DAMAGE IN CAENORHABDITIS-ELEGANS
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DOI：
10.1073/pnas.90.19.8905
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发表时间：
1993-10-01
影响因子：
11.1
通讯作者：
LARSEN, PL
中科院分区：
文献类型：
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作者：
LARSEN, PL
dauer幼虫状态和年龄-1突变，这两者都延长了秀丽隐杆线虫的寿命，测试了对可能与衰老有关的细胞损伤的超抗性。年龄-1菌株TJ 401显示相对于其亲本菌株对氧化应激的超抗性。保护细胞免受氧化损伤的两种酶，超氧化物歧化酶（SOD）和过氧化氢酶的活性在突变动物中显示出年龄依赖性的增加，这在亲本菌株中没有发现。这些活动的增加加速了超抵抗的时间进程。结果与age-1基因产物作为SOD和过氧化氢酶活性的负调节剂起作用是一致的。在野生型和年龄-1 dauer幼虫，SOD活性水平升高，但没有过氧化氢酶活性，与年轻的成年人相比。SOD活性的普遍增加促使克隆C. Cu/Zn SOD基因。它在基因组物理图谱上的位置位于age-1基因已被遗传定位的区域，但SOD基因座的突变不太可能赋予Age表型。结果支持衰老的自由基理论，表明增加抗氧化应激可能是增加寿命的原因之一，在两个菌株TJ 401和dauer幼虫。
The dauer larva state and the age-1 mutation, both of which extend life-span in Caenorhabditis elegans, were tested for hyperresistance to cellular damage that may be relevant to aging. The age-1 strain TJ401 displayed hyperresistance to oxidative stress relative to its parental strain. The activities of two enzymes that protect cells from oxidative damage, superoxide dismutase (SOD) and catalase, showed an age-dependent increase in mutant animals, which was not seen in the parental strain. These increases in activities paralleled the time course of the hyperresistance. The results are consistent with the age-1 gene product functioning as a negative regulator of SOD and catalase activities. In wild-type and age-1 dauer larvae, elevated levels of SOD activity, but not of catalase activity, were present when compared with young adults. The common increase in SOD activity prompted cloning the C. elegans Cu/Zn SOD gene. Its position on the physical map of the genome was in the region to which the age-1 gene has been genetically mapped, but it is unlikely that a mutation at the SOD locus confers the Age phenotype. Results support the free radical theory of aging by suggesting that the increased resistance to oxidative stress may be among the causes of increased longevity in both strain TJ401 and in the dauer larva.