CELLULAR-COMPONENTS OF THE IMMUNE BARRIER IN THE SPINAL MENINGES AND DORSAL-ROOT GANGLIA OF THE NORMAL RAT - IMMUNOHISTOCHEMICAL (MHC CLASS-II) AND ELECTRON-MICROSCOPIC OBSERVATIONS
CELLULAR-COMPONENTS OF THE IMMUNE BARRIER IN THE SPINAL MENINGES AND DORSAL-ROOT GANGLIA OF THE NORMAL RAT - IMMUNOHISTOCHEMICAL (MHC CLASS-II) AND ELECTRON-MICROSCOPIC OBSERVATIONS
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DOI：
10.1007/bf00312822
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发表时间：
1993-08-01
影响因子：
3.6
通讯作者：
ZENKER, W
中科院分区：
文献类型：
--
作者：
BRAUN, JS;KAISSLING, B;ZENKER, W
本文研究了正常大鼠脊膜和背根神经节中骨髓源性细胞（游离细胞）的分布、形态和特异性局部关系。用透射电镜和扫描电镜研究了这些细胞的形态。表达主要组织相容性复合体（MHC） II类基因产物的细胞已被免疫荧光识别。在透射电子显微镜下，脑膜的所有层中都有游离细胞。其中许多具有巨噬细胞特有的超微结构特征，而另一些则表现出高度空泡化的细胞质，并被赋予许多过程。这些元素缺乏明显的溶酶体系统，可能代表树突状细胞。扫描电镜显示，游离细胞通过丰富的细胞质过程接触脑脊液，这些细胞质过程穿过脑脊液和蛛网膜的细胞层。表达MHC II类抗原的细胞也存在于脑膜的所有层中。它们在紧邻蛛网膜下腔的层、硬脑膜血管附近、脊髓根和硬脑膜漏斗中尤其丰富。除脑膜外，在背根神经节中观察到对MHC II类抗原的强免疫反应性。本研究的超微结构和免疫组织化学结果表明存在一个发达的蛛网膜下腔和背根神经节的免疫监测系统。
This report deals with the distribution, morphology and specific topical relationships of bone-marrow-derived cells (free cells) in the spinal meninges and dorsal root ganglia of the normal rat. The morphology of these cells has been studied by transmission and scanning electron microscopy. Cells expressing the major histocompatibility complex (MHC) class II gene product have' been recognized by immunofluorescence. At the level of the transmission electron microscope, free cells are found in all layers of the meninges. Many of them display characteristic ultrastructural features of macrophages, whereas others show a highly vacuolated cytoplasm and are endowed with many processes. These elements lack a conspicuous lysosomal system and might represent dendritic cells. Scanning electron microscopy has revealed that free cells contact the cerebrospinal fluid via abundant cytoplasmic processes that cross the cell layers of the pia mater and of the arachnoid. Cells expressing the MHC class II antigen are also found in all layers of the meninges. They are particularly abundant in the layers immediately adjacent to the subarachnoid space, in the neighbourhood of dural vessels, along the spinal roots and in the dural funnels. In addition to the meninges, strong immunoreactivity for MHC class II antigen is observed in the dorsal root ganglia. The ultrastructural and immunohistochemical findings of this study suggest the existence of a well-developed system of immunological surveillance of the subarachnoid space and of the dorsal root ganglia.