Modulation of transcription affects mRNP quality
Modulation of transcription affects mRNP quality
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DOI：
10.1016/j.molcel.2004.09.019
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发表时间：
2004-10-22
期刊：
影响因子：
16
通讯作者：
Libri, D
中科院分区：
文献类型：
--
作者：
Jensen, TH;Boulay, J;Libri, D
蛋白质在新生转录物上的共转录负载有助于形成信使核糖核蛋白颗粒(MRNPs)，具有核出口的能力。转录机制被认为在mRNP组装中发挥关键作用，mRNP组装至少部分与Tho/TREX复合体和mRNA终止/多聚腺苷化装置的功能有关。在这里，我们展示了转录速度在生产出口能力强的mRNPs中的突出作用。我们发现，RAD3P解旋酶的转录缺陷等位基因，TFIIH转录起始因子的一种成分，抑制了与缺陷mRNA处理和输出相关的几种表型。引人注目的是，Rad3p活性受损的影响可以被一种转录延长药物以及其他影响转录的突变所表现出来。我们的结果表明，有效的mRNP组装受到转录速率影响的动力学控制。
Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/ TREX complex and the mRNA termination/polyadenylation apparatus. Here we demonstrate a prominent role for the rate of transcription in the production of export-competent mRNPs. We show that a transcription-defective allele of the Rad3p helicase, a component of the TFIIH transcription initiation factor, suppresses several phenotypes associated with defective mRNA processing and export. Strikingly, the effects of compromised Rad3p activity can be phenocopied by a transcription elongation drug as well as by other mutations affecting transcription. Our results suggest that efficient mRNP assembly is under a kinetic control that is influenced by the rate of transcription.