The viral ubiquitin gene of Autographa californica nuclear polyhedrosis virus is not essential for viral replication
The viral ubiquitin gene of Autographa californica nuclear polyhedrosis virus is not essential for viral replication
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DOI：
10.1006/viro.1996.0185
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发表时间：
1996-04-01
期刊：
影响因子：
3.7
通讯作者：
Guarino, LA
中科院分区：
文献类型：
--
作者：
Reilly, LM;Guarino, LA
加州自签名杆状病毒核型多角体病毒（AcNPV）编码一种与泛素具有显著同源性的蛋白。为了研究病毒泛素在感染中的作用，利用病毒泛素基因v-ubi编码序列的移码突变构建了重组病毒。这个被命名为vubii - fs的重组蛋白是有活力的，这表明病毒泛素在组织培养中不是复制所必需的。但在单步生长曲线上，感染性出芽病毒的产量下降了5 ~ 10倍，总出芽病毒粒子的产量也有类似程度的下降。突变病毒颗粒含有磷脂修饰形式的泛素（Pt-Ub），氨基酸序列分析显示只有宿主泛素被包装成病毒粒子。总之，这些结果表明，病毒泛素是一种非必需蛋白，在某些条件下可能具有轻微的生长优势。(C) 1996学术出版社，Inc.
The baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) encodes a protein with significant homology to ubiquitin. To study the role of viral ubiquitin in infection, a recombinant virus was constructed with a frameshift mutation within the coding sequence of the viral ubiquitin gene, v-ubi. This recombinant, named Vubi-FS, was viable, indicating that viral ubiquitin is not essential for replication in tissue culture. However, the yields of infectious budded virus were decreased 5- to 10-fold in single step growth curves, and the production of total budded virions was reduced to a similar extent. The mutant virus particles contained the phospholipid-modified form of ubiquitin (Pt-Ub), and amino acid sequence analysis revealed that only host ubiquitin was packaged into virions. Together, these results suggest that viral ubiquitin is a nonessential protein that may confer a slight growth advantage under certain conditions. (C) 1996 Academic Press, Inc.