Colorimetric sensor for cysteine in human urine based on novel gold nanoparticles
Colorimetric sensor for cysteine in human urine based on novel gold nanoparticles
复制标题
基于新型金纳米粒子的人尿液中半胱氨酸比色传感器
DOI：
10.1016/j.talanta.2016.09.009
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发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
Dong Chuan
中科院分区：
文献类型：
--
作者：
Zhang Yan;Jiang Jingjing;Li Min;Gao Pengfei;Zhou Ying;Zhang Guomei;Shuang Shaomin;Dong Chuan
本文首次报道了一种基于果胶酶保护的金纳米颗粒（P@AuNPs）的半胱氨酸（Cys）的新型、高灵敏度和特异性比色传感器。采用微波辅助加热法合成了P@AuNPs，并通过UV-vis、TEM、FT-IR和zeta电位等手段对其进行了表征。Cys通过形成强的Au-S共价键和静电结合作用，使P@AuNPs发生聚集。随着Cys浓度的增加，溶液的颜色逐渐从酒红色变为蓝色，并且P@AuNPs聚集后的大吸收带从523 nm移动到650 nm。此外，各种实验参数，如pH值，P@AuNPs的量和孵育时间进行了研究的最佳传感条件。Cys的浓度可以通过用肉眼或紫外-可见光谱仪监测来确定。所提出的比色传感器显示出极端的选择性对确定的Cys在20倍的所有其他不同的干扰物的存在下。在最佳条件下，该方法在4.85×10 - 9 ~ 3.02×10 - 4 M（R2=0.996）和3.25×10 - 3 ~ 1.03×10 - 2 M（R2=0.999）范围内具有良好的线性关系，检测限为4.6×10 - 9 M。将该传感器成功应用于人尿样中半胱氨酸的检测，证明了该传感器在生物体系中的实际应用价值。
Herein, a novel, high sensitive, and specific colorimetric sensor for cysteine (Cys) based on pectinase protected gold nanoparticles (P@AuNPs) has been demonstrated for the first time. The P@AuNPs were synthesized by “MW-assisted heat method” and were characterized by UV–vis, TEM, FT-IR and zeta potential techniques. Cys could cause the aggregation of P@AuNPs due to formation of the strong covalent Au-S bond and electrostatic binding. As the Cys concentration increased, the color of the solutions gradually changed from wine-red to blue as well as the large absorption band shifted from 523 to 650 nm upon P@AuNPs aggregation. In addition, various experimental parameters such as pH, the amount of P@AuNPs and incubation time were investigated for the optimum sensing conditions. The concentration of Cys could be determined by monitoring with the naked eye or a UV–vis spectrometer. The proposed colorimetric sensor showed an extreme selectivity toward the determination of Cys in the presence of 20-fold all other different interferents. Under optimum conditions, this method exhibited two good linear ranges from 4.85×10−9to 3.02×10−4M (R2=0.996) and 3.25×10−3to 1.03×10−2M (R2=0.999), with a low detection limit of 4.6×10−9M. Moreover, this colorimetric sensor was successfully applied to the detection of Cys in human urine samples, demonstrating its great value for practical application in biological systems.