Application of 15 N 2 -Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP
Application of 15 N 2 -Diazirines as a Versatile Platform for Hyperpolarization of Biological Molecules by d-DNP
复制标题
15 N 2 -二氮丙啶作为 d-DNP 生物分子超极化的多功能平台的应用
DOI：
10.1021/acs.bioconjchem.0c00028
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发表时间：
2020
影响因子：
4.7
通讯作者：
Wang, Qiu
中科院分区：
文献类型：
--
作者：
Park, Hyejin;Zhang, Guannan;Bae, Junu;Theis, Thomas;Warren, Warren S.;Wang, Qiu
15 N2-二氮杂环丙烷代表了一类有吸引力的超极化磁共振成像（HP-MRI）成像标签，提供了理想的生物相容性，易于掺入各种分子，并能够提供持久的极化。我们最近建立了超极化的15 N2-diazirines在有机溶剂中使用SABRE-shield使对准转移到异核（SABRE-SHEATH）。然而，目前SABRE-SHEATH在水中的挑战，特别是较差的极化效率，在检查15 N2-二氮杂环丙烷用于HP-MRI的实际用途时存在障碍。在这里，我们表明，有效的极化diverse 15 N2-diazirine标记的分子在水中可以很容易地实现溶解动态核极化（d-DNP），超极化技术在临床实践中使用。与SABRE-SHEATH相比，d-DNP的超极化也表现出对持久15 N信号的更大增强。本论文研究了氨基酸、糖和药物等多种生物重要分子，展示了15 N2-diazirines作为分子标记物在生物医学和临床应用中的巨大潜力。
15N2-Diazirines represent an attractive class of imaging tags for hyperpolarized magnetic resonance imaging (HP-MRI), offering desirable biocompatibility, ease of incorporation into a variety of molecules, and ability to deliver long-lasting polarization. We have recently established hyperpolarization of15N2-diazirines in organic solvents using SABRE-Shield Enables Alignment Transfer to Heteronuclei (SABRE-SHEATH). Yet, the current challenge of SABRE-SHEATH in water, specifically poor polarization efficiency, presents a barrier in examining the practical use of15N2-diazirines for HP-MRI. Herein, we show that efficient polarization of diverse15N2-diazirine-labeled molecules in water can be readily achieved by dissolution dynamic nuclear polarization (d-DNP), a hyperpolarization technique used in clinical practice. Hyperpolarization by d-DNP also demonstrates greater enhancement for long-lasting15N signals, in comparison with SABRE-SHEATH. Various biologically important molecules are studied in this work, including amino acid, sugar, and drug compounds, demonstrating the great potential of15N2-diazirines as molecular tags in broad biomedical and clinical applications.