Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation
Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation
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DOI：
10.1021/acssuschemeng.7b02610
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发表时间：
2018-01-01
影响因子：
8.4
通讯作者：
Takada, Jun
中科院分区：
文献类型：
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作者：
Kunoh, Tatsuki;Takeda, Minoru;Takada, Jun
金属纳米颗粒，特别是金纳米颗粒（AuNPs）的绿色合成已经吸引了医学和制药领域的科学家和工程师的极大兴趣;因此，已经开发了各种生态友好、节能和节省成本的技术。在这项研究中，我们发现，细胞的纤发菌（铁氧化细菌）释放细胞外RNA，其中一些可以存在作为一个组成部分的细胞封闭鞘。作为研究的一部分，在鞘中的金属结壳，在这里，我们表明，RNA制备的纤发菌细胞可以减少Au（III）和球形金纳米粒子最终形成时，HAuCl 4水溶液在环境条件下加入。其他生物起源的RNA和DNA也具有同样的能力。在核苷和核碱基中，只有鸟苷和鸟嘌呤可以形成AuNP。当与HAuCl 4溶液混合时，DNA部分2 '-脱氧鸟苷（dG）（用作参考材料）形成AuNP，但8-羟基-2'-脱氧鸟苷（8-OHdG）不形成，表明AuNP的形成显然取决于鸟嘌呤部分的还原电位，而不是糖部分。这一发现是第一次证明，球形金纳米粒子的ca。5 nm直径可以通过在环境温度下简单地将鸟嘌呤添加到HAuCl 4溶液中来获得;不需要其他化学或物理处理。
Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2'-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2'-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.