K-channels in an insulin-secreting cell line: effects of ATP and sulphonylureas.
K-channels in an insulin-secreting cell line: effects of ATP and sulphonylureas.
复制标题
胰岛素分泌细胞系中的 K 通道：ATP 和磺脲类药物的作用。
DOI：
10.1007/978-1-4684-5314-0_6
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发表时间：
1986
影响因子：
--
通讯作者：
Hales,CN
中科院分区：
文献类型：
--
作者：
Ashford,ML;Sturgess,NC;Cook,DL;Hales,CN
最近将膜片钳技术应用于研究β细胞质膜上的单个通道，发现了一种通道，其活性可通过在细胞膜表面应用三磷酸腺苷来抑制。有人认为，这一通道可能与葡萄糖诱导的β细胞去极化有关，而三磷酸腺苷可能是葡萄糖代谢和胰岛素分泌刺激之间长期寻求的联系。细胞系被证明是非常方便和可重复性的系统，用于研究代谢过程及其调节，我们发现它们特别适合于膜片钳程序。因此，我们利用膜片钳技术研究了一种新的胰岛素分泌细胞系5，以研究钾通道及其在控制胰岛素分泌中的作用。
The recent application of the patch-clamp technique to the study of single channels present in the β-cell plasma membrane has led to the discovery of a channel the activity of which is inhibited by the application of ATP to the membrane cytoplasmic surface7. It has been suggested that this channel may be responsible for glucose-induced β-cell depolarisation and that ATP may be the long-sought connection between glucose metabolism and the excitation of insulin secretion7,8. Cell lines are proving to be very convenient and reproducible systems with which to study metabolic processes and their regulation and we have found them particularly suitable for patch clamp procedures. We have therefore investigated a new insulin-secreting cell line5using the patch-clamp technique in order to study potassium channels and their role in the control of insulin secretion.