Ascospore inactivation and germination by high pressure processing is affected by ascospore age
Ascospore inactivation and germination by high pressure processing is affected by ascospore age
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DOI：
10.1016/j.ifset.2007.04.011
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发表时间：
2007-12-01
影响因子：
6.6
通讯作者：
Buckle, K. A.
中科院分区：
文献类型：
--
作者：
Chapman, B.;Winley, E.;Buckle, K. A.
本文研究了龄期对耐热霉菌黄丝壳霉（Byssochlamysfulva，B.）nivea、Neaportorya fischeri和N. spinosa被确定。从在麦芽提取物琼脂上在30 ℃下生长3-15周的培养物中收获子囊孢子。在过滤和测定浓度后，将子囊孢子在0.1M柠檬酸盐磷酸盐缓冲液（pH 4和6）和芒果泥（pH 5）中在600 MPa下进行高压处理（HPP）10分钟。结果支持了我们的假设，年龄（成熟度）影响耐高温霉菌的子囊孢子的耐高压性。B的3周龄子囊孢子的减少量为log（10）2.5 cfu mL（-1）。nivea，而对于9周龄的子囊孢子，仅实现了半个对数减少。对于B观察到类似的结果。nivea和N. fischeri。HPP处理引起了N. spinosa，老子囊孢子显示增加的激活。皇冠版权所有（c）2007年出版的爱思唯尔有限公司保留所有权利。
The effects of age on high pressure resistance of the ascospores of heat resistant moulds Byssochlamys fulva, B. nivea, Neosartorya fischeri and N. spinosa were determined. Ascospores were harvested from cultures grown for 3-15 weeks at 30 degrees C on malt extract agar. Following filtration and determination of concentration, the ascospores were subjected to high pressure processing (HPP) at 600 MPa for 10 min in 0.1 M citrate phosphate buffer (pH 4 and 6) and mango puree (pH 5). The results supported our hypothesis that age (maturity) affects high pressure resistance of ascospores of heat resistant moulds. A reduction of log(10) 2.5 cfu mL(-1) was achieved for three week old ascospores of B. nivea whereas for nine week old ascospores only a half log reduction was achieved. Similar results were observed for B. nivea and N. fischeri. The HPP treatment caused activation of ascospores of N. spinosa, with older ascospores showing increased activation. Crown Copyright (c) 2007 Published by Elsevier Ltd. All rights reserved.