Alkaline extraction and acid precipitation of phenolic compounds from longan (Dimocarpus longan L.) seeds
Alkaline extraction and acid precipitation of phenolic compounds from longan (Dimocarpus longan L.) seeds
复制标题
龙眼种子酚类化合物的碱提和酸沉
DOI：
10.1016/j.seppur.2014.01.030
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发表时间：
2014-03-18
影响因子：
8.6
通讯作者：
He, Ning
中科院分区：
文献类型：
--
作者：
Li, Xiangnan;Huang, Jiale;He, Ning
研究了龙眼核酚类化合物的碱提酸沉工艺，以开发一种比现有工艺更实用的工艺来分离这些化学物质。以碱性缓冲液为萃取溶剂，常规固液萃取后总酚得率达46.86 ± 0.43 mg/g。由于预浓缩过程的优点，酸沉效率达到65.29 ± 0.38%，酚类分离收率达到22.04 ± 1.95 mg/g。然而，酚类提取物的纯度受到蛋白质和碳水化合物沉淀物中存在的影响，蛋白质和碳水化合物在不同pH值下在所有四个沉淀级分中一起移动。FRAP测定和自由基清除活性测定表明，沉淀提取物中的酚类化合物具有较强的抗氧化活性。该工艺具有操作简单、产品安全、生产成本低等优点，具有良好的工业应用前景。(C)2014爱思唯尔有限公司版权所有。
The alkaline extraction and acid precipitation of longan seed phenolic compounds were investigated to develop a more practical process than currently exists to isolate these chemicals. By using an alkaline buffer as the extraction solvent, the yield of total phenols reached 46.86 +/- 0.43 mg/g after conventional solid-liquid extraction. Because of the benefit achieved from the preconcentration process, an acid precipitation efficiency of 65.29 +/- 0.38% was obtained, and the separation yield of phenols reached 22.04 +/- 1.95 mg/g. However, the purity of the phenolic extract was influenced by the presence in the precipitate of proteins and carbohydrates, which both moved together in all four precipitation fractions at different pH values. The FRAP assay and the assay of the radical-scavenging activity revealed the strong antioxidant activity possessed by the phenolic compounds in the precipitate extracts. This preparation process for phenolic compounds has obvious advantages, such as easy operation, safe products and low production cost, which indicated its prospective industrial potential. (C) 2014 Elsevier Ltd. All rights reserved.