Pregnancy specific beta 1-glycoprotein in human intestine.
Pregnancy specific beta 1-glycoprotein in human intestine.
复制标题
人肠道中妊娠特异性 β1-糖蛋白。
DOI：
10.1007/bf00926089
复制
发表时间：
1993
影响因子：
4.3
通讯作者：
Chan,WY
中科院分区：
文献类型：
--
作者：
Shupert,WL;Chan,WY
妊娠特异性 β1-糖蛋白 (PSG) 转录本已在许多胎盘和非胎盘组织中被发现。使用胎盘 PSG cDNA 探针筛选正常人肠道 cDNA 文库，我们分离出 22 个杂交克隆。这些克隆可以分为四组。核苷酸序列分析显示，一组克隆对应于功能性PSG cDNA，另一组对应于非功能性PSG cDNA。另外两组与非特异性交叉反应抗原（NCA）和胆汁糖蛋白（BGP）同源，两者都是癌胚抗原（CEA）家族的成员。因此，PSG、NCA和BGP在正常人肠道中共表达。 RNA 和免疫印迹分析以及聚合酶链反应扩增进一步证实了 PSG 在人肠道组织中的表达。 （摩尔细胞生物化学 120：159–170，1993）
Pregnancy-specific β1-glycoprotein (PSG) transcripts have been identified in a number of placental and non-placental tissues. Using a placental PSG cDNA probe to screen a normal human intestinal cDNA library we have isolated 22 hybridizing clones. These clones could be divided into four groups. Nucleotide sequence analysis showed that one group of clones correspond to functional and another group correspond to non-functional PSG cDNAs. The other two groups are homologous to the nonspecific cross-reacting antigen (NCA) and biliary glycoprotein (BGP), both of which are members of the carcinoembryonic antigen (CEA) family. Thus, PSG, NCA and BGP are co-expressed in normal human intestine. RNA and immunoblot analysis, along with polymerase chain reaction amplification further confirm the expression of PSG in human intestinal tissue. (Mol Cell Biochem120: 159–170, 1993)