Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability
Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability
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DOI：
10.1016/s0147-6513(03)00083-6
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发表时间：
2004-03-01
影响因子：
6.8
通讯作者：
Bols, NC
中科院分区：
文献类型：
--
作者：
Dayeh, VR;Chow, SL;Bols, NC
使用荧光染料的三种活力测定法有效地检测了三种哺乳动物细胞系（H4 IIE、Caco 2和HepG-2）、两种鱼类细胞系（RTgill-W1和RTL-W1）和一种纤毛原生动物嗜热四膜虫（Tetrahymena thermophila）在暴露于Triton X-100（用作模型毒物）后的活力丧失。染料为Alcohol Blue（AB）、中性红（NR）和碘化丙啶，分别监测能量代谢、溶酶体活性和膜完整性。第四种荧光染料，5-羧基荧光素二乙酸酯乙酰氧基甲酯，是有问题的。对于2小时的Triton X-100曝光，哺乳动物细胞系的鱼细胞系的敏感性，而嗜热T约为两倍的灵敏度与AB和NR检测。尽管敏感性较低，但嗜热T细胞的细胞毒性试验可以在泉水中进行，这意味着与动物细胞不同，它们可以直接暴露于大多数工业废水中，而无需进行渗透压调节。因此，嗜热T细胞可以作为动物细胞的有益补充，作为鱼类毒性测试的替代品。(C)2003年爱思唯尔公司All rights reserved.
Three viability assays using fluorescent dyes effectively detected a loss of viability in cultures of three mammalian cell lines (H4IIE, Caco2, and HepG-2), two fish cell lines (RTgill-W1 and RTL-W1), and a ciliated protozoan, Tetrahymena thermophila, after exposure to Triton X-100, used as a model toxicant. The dyes were Alamar Blue (AB), neutral red (NR), and propidium iodide, which respectively monitored energy metabolism, lysosomal activity, and membrane integrity. A fourth fluorescent dye, 5-carboxyfluorescein diacetate acetoxymethyl ester, was problematic. For 2-h Triton X-100 exposures, mammalian cell lines were as susceptible as piscine cell lines, whereas T thermophila was approximately twofold less sensitive as detected with AB and NR. Despite being less sensitive, cytotoxicity tests on T thermophila could be done in spring water which means that unlike animal cells they could be directly exposed to most industrial effluents without osmolality adjustments. Therefore, T thermophila could be a useful complement to animal cells as alternatives to fish in toxicity testing. (C) 2003 Elsevier Inc. All rights reserved.