Implementation of spin‐echo blood oxygen level‐dependent (BOLD) functional MRI in birds
Implementation of spin‐echo blood oxygen level‐dependent (BOLD) functional MRI in birds
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在鸟类中实施自旋回波血氧水平依赖性（BOLD）功能性 MRI
DOI：
10.1002/nbm.1525
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发表时间：
2010
影响因子：
2.9
通讯作者：
A. Van der Linden
中科院分区：
文献类型：
--
作者：
C. Poirier;M. Verhoye;T. Boumans;A. Van der Linden
高场磁共振成像系统的出现使得在小动物身上实施血氧水平依赖的功能磁共振成像(BOLD FMRI)成为可能。增加的磁场提高了信噪比，从而提高了空间分辨率。然而，它也增加了通常获取的梯度回波图像中的磁化率伪影。这一问题在鸣禽核磁共振中尤为突出，因为鸟类头骨中存在大量气腔。这些与T2*相关的图像伪影可以使用自旋回波BOLD功能磁共振成像绕过。在这篇文章中，我们描述了在斑马雀身上实施自旋回波功能磁共振成像，斑马雀是一种15-25 g的小型鸣禽，在鸟鸣的行为神经科学中得到了广泛的研究。由于这一研究领域的主要课题是歌曲感知和歌曲学习，因此该协议使用了听觉刺激来实现。尽管与梯度回波BOLD fMRI相比，自旋回波BOLD fMRI具有听觉特性和弱的对比度噪声比，但我们成功地检测到不同刺激触发的BOLD反应在统计学上的显著差异。这项研究表明，自旋回波BOLD fMRI是研究小型鸣禽全脑听觉处理的一种可行的方法。它也可以应用于研究其他小动物的听觉处理，以及其他感官方式。版权所有©2010 John Wiley&Sons，Ltd.
The advent of high‐field MRI systems has allowed the implementation of blood oxygen level‐dependent functional MRI (BOLD fMRI) on small animals. An increased magnetic field improves the signal‐to‐noise ratio and thus allows an improvement in the spatial resolution. However, it also increases susceptibility artefacts in the commonly acquired gradient‐echo images. This problem is particularly prominent in songbird MRI because of the presence of numerous air cavities in the skull of birds. These T2*‐related image artefacts can be circumvented using spin‐echo BOLD fMRI. In this article, we describe the implementation of spin‐echo BOLD fMRI in zebra finches, a small songbird of 15–25 g, extensively studied in the behavioural neurosciences of birdsong. Because the main topics in this research domain are song perception and song learning, the protocol implemented used auditory stimuli. Despite the auditory nature of the stimuli and the weak contrast‐to‐noise ratio of spin‐echo BOLD fMRI compared with gradient‐echo BOLD fMRI, we succeeded in detecting statistically significant differences in BOLD responses triggered by different stimuli. This study shows that spin‐echo BOLD fMRI is a viable approach for the investigation of auditory processing in the whole brain of small songbirds. It can also be applied to study auditory processing in other small animals, as well as other sensory modalities. Copyright © 2010 John Wiley & Sons, Ltd.