Prevalence of Virulence Determinants and Antimicrobial Resistance among Commensal Escherichia coli Derived from Dairy and Beef Cattle
Prevalence of Virulence Determinants and Antimicrobial Resistance among Commensal Escherichia coli Derived from Dairy and Beef Cattle
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DOI：
10.3390/ijerph120100970
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Baldy-Chudzik, Katarzyna
中科院分区：
文献类型：
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作者：
Bok, Ewa;Mazurek, Justyna;Baldy-Chudzik, Katarzyna
牛是潜在致病性大肠杆菌的宿主。大肠杆菌是一种对公众健康构成重大威胁的细菌，因此监测大肠杆菌毒力和耐药性的遗传决定因素的流行至关重要。大肠杆菌群。本研究的目的是分析大肠埃希菌的系统发育结构、毒力因子的分布和耐药性。大肠杆菌分离自两组健康牛：50头普通牛舍奶牛（147株）和42头生态牧场奶牛（118株）。系统发育分析、VF和耐药基因的鉴定均基于多重或单一PCR。对E.用微量肉汤稀释法检测大肠杆菌。使用两种统计方法分析两组牛的结果。用这两个模型描述了因变量（VF谱、抗生素）与自变量之间的关系。采用混合logit模型对分离株组中分析因子的患病率进行拟合。使用单变量逻辑回归模型来确定这些因素在特定动物中的患病率。给定每个模型，估计群体的比值比（OR）和95%置信区间。肉牛分离株以B1群为主，奶牛分离株以A群、B1群和D群为主。VFs阳性菌株的频率显着较高的分离自肉牛。E.奶牛大肠杆菌对抗生素的耐药性显著增高。一些测试的耐药基因存在于奶牛分离株中。研究表明，生境和食性可能影响了红腹锦鸡儿的遗传多样性。牛体内的大肠杆菌结果表明，生态牧场栖息地与VF的传播速率增加有关，而谷仓栖息地的特点是E.杆菌
Cattle is a reservoir of potentially pathogenic E. coli, bacteria that can represent a significant threat to public health, hence it is crucial to monitor the prevalence of the genetic determinants of virulence and antimicrobial resistance among the E. coli population. The aim of this study was the analysis of the phylogenetic structure, distribution of virulence factors (VFs) and prevalence of antimicrobial resistance among E. coli isolated from two groups of healthy cattle: 50 cows housed in the conventional barn (147 isolates) and 42 cows living on the ecological pasture (118 isolates). The phylogenetic analysis, identification of VFs and antimicrobial resistance genes were based on either multiplex or simplex PCR. The antimicrobial susceptibilities of E. coli were examined using the broth microdilution method. Two statistical approaches were used to analyse the results obtained for two groups of cattle. The relations between the dependent (VFs profiles, antibiotics) and the independent variables were described using the two models. The mixed logit model was used to characterise the prevalence of the analysed factors in the sets of isolates. The univariate logistic regression model was used to characterise the prevalence of these factors in particular animals. Given each model, the odds ratio (OR) and the 95% confidence interval for the population were estimated. The phylogroup B1 was predominant among isolates from beef cattle, while the phylogroups A, B1 and D occurred with equal frequency among isolates from dairy cattle. The frequency of VFs-positive isolates was significantly higher among isolates from beef cattle. E. coli from dairy cattle revealed significantly higher resistance to antibiotics. Some of the tested resistance genes were present among isolates from dairy cattle. Our study showed that the habitat and diet may affect the genetic diversity of commensal E. coli in the cattle. The results suggest that the ecological pasture habitat is related to the increased spreading rate of the VFs, while the barn habitat is characterised by the higher levels of antimicrobial resistance among E. coli.