PROCEDURE FOR RAPID, LARGE-SCALE PURIFICATION OF ESCHERICHIA-COLI DNA-DEPENDENT RNA-POLYMERASE INVOLVING POLYMIN-P PRECIPITATION AND DNA-CELLULOSE CHROMATOGRAPHY
PROCEDURE FOR RAPID, LARGE-SCALE PURIFICATION OF ESCHERICHIA-COLI DNA-DEPENDENT RNA-POLYMERASE INVOLVING POLYMIN-P PRECIPITATION AND DNA-CELLULOSE CHROMATOGRAPHY
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DOI：
10.1021/bi00692a011
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发表时间：
1975-01-01
期刊：
影响因子：
2.9
通讯作者：
JENDRISAK, JJ
中科院分区：
文献类型：
--
作者：
BURGESS, RR;JENDRISAK, JJ
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Richard R. Burgess* and Jerome J. Jendrisak! abstract: An improved method is described for the puri-fication of the DNA-dependent RNA polymerase [ribonucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7. 7.6] from Escherichia coli. The method involves lyso-zyme-sodium deoxycholate lysis, low-speed centrifugation, precipitation with Polymin P, elution from the Polymin PThe DNA-dependent RNA polymerase from Escherichia coli is widely used in the study of transcription. It is being purified in many laboratories by a variety of methods which vary in ease, speed, yield, reproducibility, and final purity (Babinet, 1967, Burgess, 1969, Zillig et al., 1970, Burgess and Travers, 1971, Berg et al., 1971, Niisslein and Heyden, 1972, Mukai and Iida, 1973, Humphries et al., 1973, Yar-brough and Hurwitz, 1974). We present here a procedure which is very rapid and reproducible, requires no dialysis,