In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway
In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway
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DOI：
10.1016/s0092-8674(01)00419-6
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发表时间：
2001-07-13
期刊：
影响因子：
64.5
通讯作者：
Theurkauf, WE
中科院分区：
文献类型：
--
作者：
Cha, BJ;Koppetsch, BS;Theurkauf, WE
果蝇类双核基因在哺乳细胞中合成并运输到卵母细胞，在卵母细胞中微管和外膜蛋白介导前极的定位。注射到卵母细胞内的荧光双核糖核酸显示非极性微管依赖的转运到最近的皮质表面，卵母细胞微管细胞骨架缺乏明显的轴向不对称性。尽管如此，被注射到哺育细胞细胞质、提取和注射到第二个卵母细胞中的双类信使核糖核酸显示出微管依赖的运输到前皮层。哺育细胞需要微管和外膜来支持双核mRNA的前向运输，而微管是双核mRNA-外膜颗粒共同组装所必需的。我们认为，在哺育细胞细胞质中形成的微管依赖的Exuperantia-bilike mRNA复合体允许在大体上非极性的卵母细胞微管网络上进行前特异性运输。
Drosophila bicoid mRNA is synthesized in the nurse cells and transported to the oocyte where microtubules and Exuperantia protein mediate localization to the anterior pole. Fluorescent bicoid mRNA injected into the oocyte displays nonpolar microtubule-dependent transport to the closest cortical surface, and the oocyte microtubule cytoskeleton lacks clear axial asymmetry. Nonetheless, bicoid mRNA injected into the nurse cell cytoplasm, withdrawn, and injected into a second oocyte shows microtubule-dependent transport to the anterior cortex. Nurse cells require microtubules and Exuperantia to support anterior transport of bicoid mRNA, and microtubules are required for bicoid mRNA-Exuperantia particle coassembly. We propose that microtubule-dependent Exuperantia-bicoid mRNA complex formation in the nurse cell cytoplasm allows anterior-specific transport on a grossly nonpolar oocyte microtubule network.