Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring
Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring
复制标题
母亲怀孕期间接触 1-硝基芘会增加青少年后代过敏性哮喘的易感性
DOI：
10.1016/j.chemosphere.2019.125356
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发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
De-Xiang Xu
中科院分区：
文献类型：
--
作者：
Xue Lu;Zhu-Xia Tan;Bo Wang;Jian Li;Biao Hu;Lan Gao;Hui Zhao;Hua Wang;Yuan-Hua Chen;De-Xiang Xu
1-硝基芘（1-NP）是一种典型的硝化多环芳烃，广泛存在于环境中。本研究的目的是探讨妊娠期 1-NP 暴露对后代过敏性哮喘易感性的影响。母鼠在整个怀孕期间通过灌胃暴露于1-NP（100μgkg−1）。幼犬分别在出生后第 23、29 和 36 天（PND）注射卵清蛋白（OVA）来致敏。最后一次注射后 7 天，致敏小鼠暴露于气溶胶 OVA。正如所料，OVA致敏幼仔的肺部存在相当多的炎症细胞，并伴有支气管壁增厚和充血。在 OVA 致敏幼犬的气道中观察到杯状细胞升高和粘液分泌过多。有趣的是，妊娠期 1-NP 暴露加剧了 OVA 致敏后代中炎症细胞（主要是嗜酸性粒细胞）的浸润。虽然对气道平滑肌层增厚和基底膜纤维化影响不大，但妊娠期1-NP暴露加重了OVA致敏和攻击子代气道杯状细胞增生、Muc5acmRNA上调和粘液分泌。从机制上讲，妊娠期 1-NP 暴露加剧了 OVA 致敏幼仔肺部 IL-5 的升高。这些发现表明妊娠期 1-NP 暴露会增加后代过敏性哮喘的易感性。
1-nitropyrene (1-NP) is widespread in the environment, as a typical nitrated polycyclic aromatic hydrocarbon. The purpose of this research was to explore the effects of gestational 1-NP exposure on susceptibility of allergic asthma in offspring. Maternal mice were exposed to 1-NP (100 μg kg−1) by gavage throughout the whole pregnancy. Pups were sensitized by injecting with ovalbumin (OVA) on postnatal day (PND)23, 29, and 36, respectively. At 7 days following the last injection, sensitized mice were exposed to aerosol OVA. As expected, there were quite a few inflammatory cells in the lungs of OVA-sensitized pups, accompanied by bronchial wall thickening and hyperemia. Elevated goblet cells and overproduced mucus were observed in the airways of OVA-sensitized pups. Interestingly, gestational 1-NP exposure aggravated infiltration of inflammatory cells, mainly eosinophils, in OVA-sensitized offspring. Although it had little effect on airway smooth muscle layer thickening and basement membrane fibrosis, gestational 1-NP exposure aggravated goblet cell hyperplasia,Muc5acmRNA upregulation, and mucus secretion in the airways of OVA-sensitized and challenged offspring. Mechanistically, gestational 1-NP exposure aggravated elevation of pulmonary IL-5 in OVA-sensitized pups. These findings suggest that gestational 1-NP exposure increases susceptibility of allergic asthma in offspring.