GAGA factor and TBF1 bind DNA elements that direct ubiquitous transcription of the Drosophila alpha 1-tubulin gene.
GAGA factor and TBF1 bind DNA elements that direct ubiquitous transcription of the Drosophila alpha 1-tubulin gene.
复制标题
GAGA 因子和 TBF1 结合 DNA 元件，指导果蝇 α 1-微管蛋白基因的普遍转录。
DOI：
10.1093/nar/22.22.4712
复制
发表时间：
1994
影响因子：
14.9
通讯作者：
Wensink,PC
中科院分区：
文献类型：
--
作者：
O'Donnell,KH;Wensink,PC
Three DNA regions (TE1, TE2 and the intron) regulate the ubiquitous expression of the α1-tubulin gene ofDrosophila melanogaster. In this report, we identify two proteins that bind these DNA regions. One is the previously characterized GAGA transcription factor and the other is a newly identified 62 kDa polypeptide, TBF1 (TE1-binding factor 1). Purified GAGA factor binds three sites in TE2 and at least three in the intron. TBF1 was purified from embryos and binds to both TE1 and TE2. Together, the two proteins produce the same DNase I footprints in TE1 and TE2 as does a nuclear extract that transcribes the gene accurately. These footprints cover most of the TE1 and TE2 DNA. Moreover, one binding site for each protein coincides with a site that activates transcriptionin vitro. The characteristics of the GAGA factor and the genes it regulates suggest roles these two proteins are likely to play in regulating ubiquitous expression.
