Disc degeneration in the rabbit: A biochemical and radiological comparison between four disc injury models
Disc degeneration in the rabbit: A biochemical and radiological comparison between four disc injury models
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DOI：
10.1097/01.brs.0000149191.02304.9b
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发表时间：
2005-01-01
期刊：
影响因子：
3
通讯作者：
Hutton, WC
中科院分区：
文献类型：
--
作者：
Kim, KS;Yoon, ST;Hutton, WC
研究设计.通过两个实验对4种椎间盘损伤模型进行了生化和放射学比较，以产生兔椎间盘退变。目的：建立一种可靠的椎间盘退变动物模型。为了研究延缓或预防椎间盘退变的各种干预措施，需要可靠的椎间盘退变动物模型。第一个实验：7只新西兰白色家兔（1岁，3.5- 4.5kg体重），分别建立4种不同的椎间盘损伤模型;喜树碱椎间盘内注射在L2-L3处使用23号针进行细胞核穿刺（凋亡剂），在L3-L4处使用21号针进行细胞核抽吸，在L4-L5处使用21号针进行3次纤维环穿刺，在L5-L 6处使用18号针进行1次环穿刺。L1-L2水平用作对照。12周后处死兔子。腰椎磁共振图像采用4级椎间盘退变进行评估。测量了核的含水量。二甲基亚甲基蓝（DMMB）法测定硫酸化糖胺聚糖含量。第二个实验：在第一个实验中被认为在产生椎间盘退变方面最有效的21号3-穿刺和18号1-穿刺模型再次用于第二个研究。8周后处死6只家兔，测定水分和硫酸化糖胺聚糖含量。在第一个实验中，抽吸和穿刺模型中的含水量显著降低。与对照组相比，抽吸模型中仅硫酸化糖胺聚糖含量显示出显著降低。椎间盘高度和磁共振分级记录了抽吸和穿刺模型中发生的显著变性。在第二个实验中，在21号3穿刺模型中，含水量显示出显著降低，而硫酸化糖胺聚糖含量的结果与对照数据相比均未显示出显著差异。在第一个实验中，21号3穿刺和18号1穿刺模型在兔腰椎中产生最一致的椎间盘退变。当在第二个实验中再次研究这两个模型时，21号3穿刺技术在较短时间内产生椎间盘退变方面具有上级优势。
Study Design. A biochemical and radiologic comparison of 4 disc injury models to produce disc degeneration in the rabbit was carried out in 2 experiments.Objectives. To develop a reliable animal model of intervertebral disc degeneration.Summary of Background Data. In order to study various interventions for retarding or preventing disc degeneration, a reliable animal model of disc degeneration is needed.Methods. First experiment: 7 New Zealand white rabbits (1 year old, 3.5-4.5 kg body weight) were used to test 4 different disc injury models; intradiscal injection of Camptothecin (an apoptotic agent) using a 23-gauge needle at L2-L3, nucleus aspiration using a 21-gauge needle at L3-L4, 3 anulus punctures using a 21-gauge needle at L4-L5, and 1 anulus puncture using a 18-gauge needle at L5-L6. The L1-L2 level was used as a control. Rabbits were killed 12 weeks later. Lumbar spinal magnetic resonance images were assessed using 4 grades of disc degeneration. The water content of the nucleus was measured. Dimethylmethylene blue (DMMB) assay was used to measure the sulfated-glycosaminoglycan content. Second experiment: the 21-gauge 3-puncture and the 18-gauge 1-puncture models, thought most effective in producing disc degeneration in the first experiment, were again used in a second study. Six rabbits were killed 8 weeks later, the water and sulfated-glycosaminoglycan contents being measured as in the first experiment.Results. In the first experiment, the water content in the aspiration and puncture models was significantly decreased. Only the sulfated-glycosaminoglycan content in the aspiration model showed a significant decrease as compared to the control. Disc heights and magnetic resonance grades documented significant degeneration occurring in the aspiration and puncture models. In the second experiment, the water content showed a significant decrease in the 21-gauge 3-puncture model, whereas neither of the results for the sulfated-glycosaminoglycan-content showed a significant difference as compared to the control data.Conclusion. In the first experiment, the 21-gauge 3-puncture and the 18-gauge 1-puncture models produced the most consistent disc degeneration in the rabbit lumbar spine. When these 2 models were again studied in the second experiment, the 21-gauge 3-puncture technique was superior in producing disc degeneration over a shorter period of time.