Lincomycin-Induced Secondary Metabolism in Streptomyces lividans 66 with a Mutation in the Gene Encoding the RNA Polymerase Beta Subunit
Lincomycin-Induced Secondary Metabolism in Streptomyces lividans 66 with a Mutation in the Gene Encoding the RNA Polymerase Beta Subunit
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DOI：
10.1007/s00284-020-02126-9
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发表时间：
2020-07
影响因子：
2.6
通讯作者：
Keiichiro Mukai;Momoko Kobayashi;Kanata Hoshino;T. Maruyama;Daiki Hayashi;Ryoko Hamauzu;T. Hosaka
中科院分区：
文献类型：
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作者：
Keiichiro Mukai;Momoko Kobayashi;Kanata Hoshino;T. Maruyama;Daiki Hayashi;Ryoko Hamauzu;T. Hosaka
激活链霉菌菌株产生次级代谢产物的遗传潜力可以提高有用的生物活性化合物的产量，并促进新的生物活性化合物的发现。在这项研究中，我们发现在rpoB中携带R440H突变的变铅青链霉菌，编码RNA聚合酶β亚基，在林可霉素的存在下生长，在低于最低抑菌浓度（MIC）的浓度下产生大量的放线菌紫素和某些隐蔽的次级代谢产物，尽管培养条件限制了野生型菌株的生产。结果表明，林可霉素在低于MIC的浓度下可强烈增强携带特异性突变的链霉菌产生次级代谢产物。在这项研究中，我们报告了某些rpoB突变的积极影响和对林可霉素的浓度依赖性反应对S. lividans次级代谢的积极影响相结合引起的一个有趣的现象66，并讨论了机制及其在探索链霉菌中神秘次级代谢物产生方面的适用性。
Activating the genetic potential ofStreptomycesstrains to produce secondary metabolites can improve the production of useful biologically active compounds and facilitate the discovery of novel biologically active compounds. In this study, we found thatStreptomyces lividanscarrying the R440H mutation inrpoB, encoding the RNA polymerase beta subunit, grown in the presence of lincomycin at concentrations below the minimum inhibitory concentration (MIC) produced abundant amounts of actinorhodin and certain cryptic secondary metabolites despite culture conditions that restrict their production by the wild-type strain. The results indicate that lincomycin at concentrations below the MIC may strongly potentiate secondary metabolite production byStreptomycesstrains carrying a specificrpoBmutation. In this study, we report an interesting phenomenon induced by combining the positive effects of certainrpoBmutations and concentration-dependent responses to lincomycin on secondary metabolism inS. lividans66 and discuss the mechanisms and their applicability in exploring cryptic secondary metabolite production in streptomycetes.