Genetic analysis of populations of threatened snake species using RAPD markers
Genetic analysis of populations of threatened snake species using RAPD markers
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使用 RAPD 标记对受威胁蛇种种群进行遗传分析
DOI：
10.1111/j.1365-294x.1994.tb00072.x
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发表时间：
1994
影响因子：
4.9
通讯作者：
P. J. Weatherhead
中科院分区：
文献类型：
--
作者：
H. Gibbs;K. Prior;P. J. Weatherhead
蛇是一个特别受威胁的脊椎动物类群，许多物种和种群的分布变得越来越分散。目前，很少有人知道的遗传分化的程度，存在于孤立的群体之间，即使这样的信息可能是至关重要的生存和保护。作为最近开发的RAPD遗传标记如何用于保护遗传学的一个例子，我们提出了一项研究的初步结果，该研究使用这些基于DNA的标记来评估两种受威胁的加拿大蛇，黑鼠蛇（Elaphe o.（3）东部massasauga响尾蛇（Sistrurus c. catenatus）。我们目前的信息水平的变化和可靠性的扩增产生的片段从五个引物。然后，我们使用最近开发的分析技术来估计种群内的核苷酸多样性水平和种群间的序列差异。我们的研究结果表明，由Clark & Lanigan（Molecular Biology and Evolution 1993，10，1096-1111）的方法估计的种群内差异水平接近脊椎动物mtDNA的差异，并且当使用随机化程序进行测试时，蛇种群之间的多样性很小并且不显著。因此，我们的研究提供了一个如何将RAPD应用于脊椎动物保护遗传学研究的例子，并表明我们研究的蛇种群最近才被隔离，从保护的角度来看，可能被认为是遗传等效的，尽管这一结论需要用其他基于DNA的标记来证实。
Snakes are a particularly threatened vertebrate taxon, with distributions of many species and populations becoming increasingly fragmented. At present, little is known about the degree of genetic differentiation that exists between isolated populations even though such information may be critical to their survival and conservation. As an example of how recently developed RAPD genetic markers can be used in conservation genetics, we present preliminary results from a study which used these DNA‐based markers to assess population divergence in two threatened Canadian snakes, the black rat snake (Elaphe o. obsoleta) and the eastern massasauga rattlesnake (Sistrurus c. catenatus). We present information on the levels of variation and reliability of amplification for fragments generated from five primers. We then use a recently developed analytical technique to estimate levels of nucleotide diversity within populations and sequence divergence between populations. Our results show that intrapopulation levels of divergence as estimated by the methods of Clark & Lanigan (Molecular Biology and Evolution 1993, 10, 1096–1111) approximate those found for mtDNA in vertebrates and that diversity between snake populations is small and non‐significant when tested using randomization procedures. Thus, our study provides an example of how RAPDs can be applied to conservation genetic studies of vertebrates and suggest that the snake populations we examined have only recently become isolated and maybe considered genetically equivalent from a conservation perspective, although this conclusion needs to be confirmed with other DNA‐based markers.
用 RAPD 估计核苷酸分歧的前景。
影响因子：
10.7
作者：
Clark,AG;Lanigan,CM
通讯作者：
Lanigan,CM