THE SEQUENCE (DC-DA)N.(DG-DT)N FORMS LEFT-HANDED Z-DNA IN NEGATIVELY SUPERCOILED PLASMIDS
THE SEQUENCE (DC-DA)N.(DG-DT)N FORMS LEFT-HANDED Z-DNA IN NEGATIVELY SUPERCOILED PLASMIDS
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DOI：
10.1073/pnas.80.7.1821
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
RICH, A
中科院分区：
文献类型：
--
作者：
NORDHEIM, A;RICH, A
Z-DNA-specific antibodies were used to demonstrate the formation of left-handed Z-DNA in sequences of (dC-dA)32.cntdot.dG-dT)32 in negatively supercoiled plasmids. Z-DNA was found to form at physiological negative superhelical densities (.sigma. = -0.05) in the absence of high salt concentration or chemical modifications. The binding site of the antibody to the DNA sequences was demonstrated by crosslinking experiments. (dC-dA)n.cntdot.(dG-dT)n sequences are widespread in eukaryotic DNA, and possible roles for such Z elements in chromatin activation or genome rearrangements are suggested.
