Wound healing effects of gingival fibroblasts cultured in animal-free medium
Wound healing effects of gingival fibroblasts cultured in animal-free medium
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DOI：
10.1111/j.1601-0825.2010.01654.x
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发表时间：
2010-07-01
期刊：
影响因子：
3.8
通讯作者：
Kamata, N.
中科院分区：
文献类型：
--
作者：
Nishi, H.;Ohta, K.;Kamata, N.
目的：本研究的目的是开发一种由牙龈成纤维细胞培养在无动物培养基（HFETH）制成的移植材料。方法：我们研究了人血清（HS）对牙龈成纤维细胞生长和伤口愈合能力的影响，通过细胞因子的产生来证明，在HFETH中培养的牙龈成纤维细胞。结果：人牙龈成纤维细胞在含2%HS的HFR 4培养基中培养后，其增殖能力明显增强，而在含2%HS的HFR 4培养基中培养的人牙龈成纤维细胞增殖能力明显增强。伤口愈合细胞因子，包括转化生长因子-β，角质形成细胞生长因子，肝细胞生长因子，血管内皮生长因子，和牙龈成纤维细胞产生的IL-6的增加，通过添加2%的HS的HFFERENT。此外，牙龈成纤维细胞培养在HFR 4与2%HS改善小鼠皮肤缺损的伤口愈合，以及那些培养在Dulbecco的改良Eagle的培养基与10%胎牛血清CONCLUSION：牙龈成纤维细胞培养在HFR 4与2%HS可能是有用的作为移植材料用于重建。口腔疾病（2010）16，438-444
OBJECTIVE: The purpose of this study was to develop a graft material made of gingival fibroblasts cultured in animal-free medium (HFDMI).METHODS: We examined the effects of human serum (HS) on cell growth and wound healing capability, demonstrated by cytokine production, of gingival fibroblasts cultured in HFDMI. Subsequently, the capability of fibroblasts cultured in HFDMI with 2% HS to promote the healing of skin defects was evaluated using nude mice.RESULTS: The proliferation of human gingival fibroblasts was increased when HS at a concentration of 0.5-2% was added to HFDMI. Wound healing cytokines, including transforming growth factor-beta, keratinocyte growth factor, hepatocyte growth factor, vascular endothelial growth factor, and IL-6 produced by gingival fibroblasts were increased by adding 2% HS to HFDMI. In addition, gingival fibroblasts cultured in HFDMI with 2% HS improved wound healing of mouse skin defects as well as those cultured in Dulbecco's modified Eagle's medium with 10% fetal calf serum.CONCLUSION: Gingival fibroblasts cultured in HFDMI with 2% HS may be useful as a graft material for reconstruction. Oral Diseases (2010) 16, 438-444