CRYSTAL-STRUCTURE OF A YEAST TBP TATA-BOX COMPLEX
CRYSTAL-STRUCTURE OF A YEAST TBP TATA-BOX COMPLEX
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DOI：
10.1038/365512a0
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发表时间：
1993-10-07
期刊：
影响因子：
64.8
通讯作者：
SIGLER, PB
中科院分区：
文献类型：
--
作者：
KIM, YC;GEIGER, JH;SIGLER, PB
TATA 盒与酵母 TBP 复合物的 2.5 埃晶体结构表明，TATA 盒的 8 个碱基对通过向主凹槽弯曲而与 TBP 的凹面结合，其强度前所未有。这会产生一个宽阔、缠绕的浅小凹槽，与 TBP 鞍座的整个下表面形成主要疏水界面。剧烈的弯曲和正向的扭动从根本上改变了侧翼 B 型 DNA 的轨迹。
The 2.5 angstrom crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.