MaSERK1 Gene Expression Associated with Somatic Embryogenic Competence and Disease Resistance Response in Banana (Musa spp.)
MaSERK1 Gene Expression Associated with Somatic Embryogenic Competence and Disease Resistance Response in Banana (Musa spp.)
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MaSERK1 基因表达与香蕉（Musa spp.）体细胞胚胎发生能力和抗病反应相关
DOI：
10.1007/s11105-009-0150-z
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发表时间：
2010-06-01
影响因子：
2.1
通讯作者：
Huang, Xue-Lin
中科院分区：
文献类型：
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作者：
Huang, Xia;Lu, Xiao-Yan;Huang, Xue-Lin
香蕉体细胞胚胎发生受体样激酶（SERK）基因MaSERK1是从香蕉品种Musa acuminata cv. Mas（AA）。该基因编码628个氨基酸，与已报道的椰子、水稻、玉米、拟南芥、胡萝卜、蒺藜苜蓿等植物的蛋白质序列同源性在82%以上。MaSERK1在雄花序中表达较弱，在雄花序的非胚性愈伤组织中不表达，但在雄花序的胚性愈伤组织和胚性细胞悬浮液中表达较高。在ECS的继代培养过程中，MaSERK1的表达和体细胞胚胎发生的频率受到继代培养持续时间的影响，其中表达在继代培养的0至6天内降低，在继代培养后12天增加至最高水平，此后下降。ECS的体细胞胚胎发生频率与MaSERK1转录水平呈正相关。此外，MaSERK1在香蕉（Musa paradisiaca L.）CV.东莞大蕉（ABB）是已知的抗尖孢镰刀菌（Fusarium oxysporum f.）用外源水杨酸（SA）或接种FOC 4号生理小种，可诱导产生一个新的黄瓜枯萎病菌（FOC 4号）小种。然而，MaSERK1在粘液属植物叶中的表达水平低于在其它植物叶中的表达水平。CV.已知对FOC 4号小种敏感的Pisang awak（ABB）在两种治疗后均未发生变化。这些结果表明，MaSERK1不仅可以作为香蕉体细胞胚胎发生的分子标记，而且在香蕉抗病反应中也发挥了一定的作用。
A banana somatic embryogenesis receptor-like kinase (SERK) gene, designated as MaSERK1, was isolated from Musa acuminata cv. Mas (AA). It encoded a protein of 628 amino acids with above 82% identities to reported SERKs of coconut, rice, maize, Arabidopsis, carrot, and Medicago truncatula. MaSERK1 was expressed weakly in male flower clusters, but not in male flower-derived nonembryogenic calli, but it was highly expressed in male flower-derived embryogenic calli and embryogenic cell suspensions (ECS). During subculture of ECS, MaSERK1 expression and frequency of somatic embryogenesis were influenced by the duration of subculture, wherein expression decreased within 0 to 6 days of subculture, increased to highest levels at 12 days following subculture, and dropped thereafter. The frequency of somatic embryogenesis of ECS positively correlated with MaSERK1 transcript levels. Moreover, MaSERK1 expression in leaves of Musa paradisiaca L. cv. Dongguan Dajiao (ABB), known to be resistant to Fusarium oxysporum f. sp. cubense race 4 (FOC race 4), was induced by exogenous salicylic acid (SA) or inoculation with FOC race 4. However, MaSERK1 expression levels in leaves of Mucus spp. cv. Pisang awak (ABB), known to be susceptible to FOC race 4, did not change following either treatment. These results suggested that MaSERK1 not only could serve as a molecular marker for banana somatic embryogenesis, but also played a role in disease resistance response in banana.